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anti-Mouse (Murine) NFKBIZ antibody for Flow Cytometry

Recommended NFKBIZ Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ) Antibodies
  • AA408868
  • IKBZ
  • INAP
  • MAIL
  • Mail
  • NFKBIZ
  • RGD1310834
Reactivity
Mouse (Murine)
30
27
13
Host
Rat
37
4
1
Clonality
Monoclonal
Conjugate
This NFKBIZ antibody is conjugated to PerCP-eFluor® 710
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
25
11
8
4
4
4
1
1
Supplier
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Catalog No. ABIN2680246
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Top referenced anti-NFKBIZ antibody for Flow Cytometry

Similar anti-NFKBIZ Antibodies

Application / Reactivity Mouse (Murine)
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General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ) Antibodies
Reactivity Mouse (Murine)
(30), (27), (13)
Host Rat
(37), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIZ antibody is conjugated to PerCP-eFluor® 710
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(25), (11), (8), (4), (4), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIZ Antibody

Target Details NFKBIZ Application Details Handling References for anti-NFKBIZ Antibody (ABIN2680246) Images
Specificity The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with RORa and RORgt to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.
Characteristics The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with RORa and RORgt to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.
Synonyms: Synonyms: IKBZ, INAP, MAIL, NF-kappa-B inhibitor zeta, Nfkbiz
Clone LK2NAP
Isotype IgG2a kappa

Target Details NFKBIZ

Product Details anti-NFKBIZ Antibody Application Details Handling References for anti-NFKBIZ Antibody (ABIN2680246) Images back to top
Antigen
Alternative Name IkB zeta (NFKBIZ Antibody Abstract)
Pathways NF-kappaB Signaling

Application Details

Product Details anti-NFKBIZ Antibody Target Details NFKBIZ Handling References for anti-NFKBIZ Antibody (ABIN2680246) Images back to top
Application Notes Applications reported: This LK2NAP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This LK2NAP antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set and protocol. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2 % formaldehyde overnight at 4 °C, but please evaluate for alternative fixation protocols.
Restrictions For Research Use only

Handling

Product Details anti-NFKBIZ Antibody Target Details NFKBIZ Application Details References for anti-NFKBIZ Antibody (ABIN2680246) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-NFKBIZ Antibody (ABIN2680246)

Product Details anti-NFKBIZ Antibody Target Details NFKBIZ Application Details Handling Images back to top
Product cited in:

Miyake, Satoh, Kato, Matsushita, Kumagai, Vandenbon, Tani, Muta, Akira, Takeuchi: "IκBζ is essential for natural killer cell activation in response to IL-12 and IL-18." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 41, pp. 17680-5, 2010

Images

Product Details anti-NFKBIZ Antibody Target Details NFKBIZ Application Details Handling References for anti-NFKBIZ Antibody (ABIN2680246) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-NFKBIZ antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta)  (PerCP-eFluor® 710) (ABIN2680246) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ) antibody (PerCP-eFluor® 710)