anti-Human NGFB antibody for Functional Studies

Recommended NGFB Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor beta (NGFB) Antibodies
  • NGFB
  • ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
  • hsan5
  • beta-ngf
  • Ngfb
  • Beta-NGF
  • HSAN5
  • beta-NGF
  • nerve growth factor (beta polypeptide)
  • beta-Nerve growth factor
  • nerve growth factor
  • NGF
  • ngf
  • FPV072
  • FPV076
  • Ngf
Reactivity
Human
149
88
65
11
4
3
3
1
Host
Rabbit
111
56
10
3
2
2
Clonality
Polyclonal
Conjugate
This NGFB antibody is un-conjugated
17
6
6
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
114
54
29
16
13
12
10
7
4
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116192
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116191 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 1
1 ABIN318395 Func EIA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN264353 EIA Func IP Mouse IgG1 Log in to see AS18
1 ABIN187625 EIA Func Mouse IgG1 Log in to see AS21
1 ABIN264355 EIA Func Mouse IgG1 Log in to see AS22
1 ABIN264354 EIA Func Mouse IgG1 Log in to see AS21
1 ABIN318389 Func EIA Rabbit IgG Log in to see Polyclonal 1
1 ABIN181153 EIA Func WB Mouse IgG2b Log in to see
1 ABIN596746 Func ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN210628 Func IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN233531 Func IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN209105 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN293936 Func IHC ELISA WB Sheep IgG Log in to see Polyclonal
1 ABIN293938 Func ELISA WB Sheep IgG Log in to see Polyclonal
1 ABIN210221 Func ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN576606 Func ELISA Rabbit IgG Log in to see Polyclonal

Top referenced anti-NGFB antibody for Functional Studies

Similar anti-NGFB Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 4 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cell Culture (CC) 1 Antibodies
ELISA 93 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Functional Studies (Func) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Neutralization (Neut) 28 Antibodies

General

Antigen Nerve Growth Factor beta (NGFB) Antibodies
Reactivity Human
(149), (88), (65), (11), (4), (3), (3), (1)
Host Rabbit
(111), (56), (10), (3), (2), (2)
Clonality
Polyclonal
Conjugate This NGFB antibody is un-conjugated
(17), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(120), (114), (54), (29), (16), (13), (12), (10), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NGFB Antibody

Target Details NGFB Application Details Handling ProductDetails: References for anti-NGFB antibody (ABIN116192) Images
Specificity Specific for Human beta NGF (Nerve Growth Factor). Other species not tested.
Purification Affinity Chromatography employing immobilized Human beta-NGF matrix
Immunogen Highly pure (>98%) E.coli derived recombinant Human beta-NGF.

Target Details NGFB

Product Details anti-NGFB Antibody Application Details Handling ProductDetails: References for anti-NGFB antibody (ABIN116192) Images back to top
Antigen
Alternative Name NGF (NGFB Antibody Abstract)
Background Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB
Gene ID 4803
NCBI Accession NP_002497
UniProt P01138
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size

Application Details

Product Details anti-NGFB Antibody Target Details NGFB Handling ProductDetails: References for anti-NGFB antibody (ABIN116192) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman beta NGF (1 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody isrequired. ELISA: To detect human beta NGF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human beta NGF. Western Blot: To detect human beta NGF by Western Blot analysis this antibody can beused at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant human beta NGF is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NGFB Antibody Target Details NGFB Application Details ProductDetails: References for anti-NGFB antibody (ABIN116192) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

ProductDetails: References for anti-NGFB antibody (ABIN116192)

Product Details anti-NGFB Antibody Target Details NGFB Application Details Handling Images back to top
Background publications

Miknyoczki, Wan, Chang, Dobrzanski, Ruggeri, Dionne, Buchkovich: "The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 6, pp. 1924-31, 2002