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anti-Mouse (Murine) NGFB antibody for Dot Blot

Recommended NGFB Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor beta (NGFB) Antibodies
  • Beta-NGF
  • beta-NGF
  • beta-ngf
  • HSAN5
  • hsan5
  • ngfb
  • NGFB
  • Ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
Reactivity
Rat (Rattus), Mouse (Murine), Human
132
83
49
9
4
3
Host
Rabbit
117
54
10
4
2
2
Clonality
Polyclonal
Conjugate
This NGFB antibody is un-conjugated
19
8
7
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
123
115
53
25
18
13
11
10
6
4
3
2
2
1
Supplier
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Catalog No. ABIN1720891
$ 500.00
Plus shipping costs $45.00

Similar anti-NGFB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 37 Antibodies 61 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 12 Antibodies 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 3 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Western Blotting (WB) 27 Antibodies
Blocking Reagent (BR) 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Functional Studies (Func) 14 Antibodies

General

Antigen Nerve Growth Factor beta (NGFB) Antibodies
Reactivity Rat (Rattus), Mouse (Murine), Human
(132), (83), (49), (9), (4), (3)
Host Rabbit
(117), (54), (10), (4), (2), (2)
Clonality
Polyclonal
Conjugate This NGFB antibody is un-conjugated
(19), (8), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(123), (115), (53), (25), (18), (13), (11), (10), (6), (4), (3), (2), (2), (1)
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Product Details anti-NGFB Antibody

Target Details NGFB Application Details Handling Images
Cross-Reactivity (Details) Not reactive in: bovine
Purification Protein G purification
Immunogen Native mouse beta NGF purified from submaxillary salivary gland.
Isotype IgG

Target Details NGFB

Product Details anti-NGFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name NGF (NGFB Antibody Abstract)
Background Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Homodimer, associated by noncovalent forces. It belongs to the NGF-beta family.
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-NGFB Antibody Target Details NGFB Handling Images back to top
Application Notes Recommended Dilution: 1-3 µg/mL (IHC, western blot, immunoblot and ELISA). Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500 ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Optimal dilutions is determined by the end user.
Restrictions For Research Use only

Handling

Product Details anti-NGFB Antibody Target Details NGFB Application Details Images back to top
Format Lyophilized
Handling Advice Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Storage 4 °C
Storage Comment After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent.