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anti-Mouse (Murine) NGFB antibody for Immunocytochemistry

Recommended NGFB Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor beta (NGFB) Antibodies
  • Beta-NGF
  • beta-NGF
  • beta-ngf
  • HSAN5
  • hsan5
  • ngfb
  • NGFB
  • Ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
Epitope
C-Term
9
7
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
134
85
51
9
4
3
Host
Rabbit
118
55
10
4
2
2
Clonality
Monoclonal
Conjugate
This NGFB antibody is un-conjugated
19
8
7
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
125
116
54
26
18
13
11
10
6
4
3
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN189595
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Similar anti-NGFB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 38 Antibodies 62 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 12 Antibodies 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 3 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 27 Antibodies
Blocking Reagent (BR) 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Functional Studies (Func) 14 Antibodies

General

Antigen Nerve Growth Factor beta (NGFB) Antibodies
Epitope C-Term
(9), (7), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(134), (85), (51), (9), (4), (3)
Host Rabbit
(118), (55), (10), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NGFB antibody is un-conjugated
(19), (8), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(125), (116), (54), (26), (18), (13), (11), (10), (6), (4), (3), (2), (2), (1), (1), (1)
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Product Details anti-NGFB Antibody

Target Details NGFB Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near the C-terminus of human NGF-Beta was used as an immunogen.
Clone EP1320Y
Isotype IgG

Target Details NGFB

Product Details anti-NGFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name NGF (NGFB Antibody Abstract)
Background Nerve growth factor (NGF) stimulates division and differentiation of sympathetic andembryonic sensory neurons. NGF is thought to have a profound effect on thedevelopment and maintenance of sympathetic and embryonic sensory neurons (1). NGFactivity isolated from the male mouse submaxillary gland (MSG) consists of three typesof subunits, alpha, beta and gamma, which specifically interact to form a 7S,approximately 130,000-molecular weight (Mr) complex. The 7S complex contains twoidentical 118-amino acid beta-chains, which are solely responsible for the nervegrowth-stimulating activity of NGF. While NGF is found in almost all vertebrates, mostresearch has focused on murine NGF, as the mouse male submaxillary gland containshigher levels of this polypeptide than other tissues (2). Alternate Names: anti-Beta nerve growth factor precursor antibody, anti-Beta NGF antibody, anti-HSAN5antibody, anti-Nerve growth factor beta polypeptide antibody, anti-NGF antibody,anti-NGFB antibody.
Gene Symbol: NGF
Gene ID 4803, 18049, 310738
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-NGFB Antibody Target Details NGFB Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry. In Western blot this antibody detects a band at approximately 32kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-100, Western Blot 1:1000-10000
Protocol Protocol specific for NGF Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-NGFB Antibody Target Details NGFB Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-NGFB Antibody Target Details NGFB Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NGFB antibody (Nerve Growth Factor beta) (C-Term) (ABIN189595) Western blot analysis on fetal thyroid tissue using anti-NGF-? RabMAb (cat. # 110-572...
Immunohistochemistry (IHC) image for anti-NGFB antibody (Nerve Growth Factor beta) (C-Term) (ABIN189595) Immunohistochemical staining of paraffin-embedded human brain using anti-NGF-? RabMAb...