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Human NGFB ELISA Kit

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Antigen
Nerve Growth Factor beta (NGFB) ELISA Kits
  • Beta-NGF
  • beta-NGF
  • beta-ngf
  • HSAN5
  • hsan5
  • ngfb
  • NGFB
  • Ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
16
13
10
4
3
3
2
2
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2506945
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32334948 ABIN414909 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 2
0.32334948 ABIN411335 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see 5
0.32334948 ABIN924756 Sandwich ELISA Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
0.32334948 ABIN1888474 Sandwich ELISA Serum, Plasma (heparin), Cell Culture Supernatant, Urine 14-5000 pg/mL 14 pg/mL Log in to see
0.0012145225 ABIN3032893 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 6.9-5000 pg/mL 6.9 pg/mL Log in to see
0.0012145225 ABIN3198504 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012145225 ABIN624954 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 4
0.0012145225 ABIN455962 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2685070 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
0.0012145225 ABIN1029366 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012145225 ABIN1112764 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2702851 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 40-10.000 pg/mL 40 pg/mL Log in to see
0.0012145225 ABIN2113132 Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
0.0012145225 ABIN1847017 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012145225 ABIN2945653 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012145225 ABIN2116279 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced NGFB ELISA Kit for ELISA

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Application / Reactivity Human
ELISA 17 ELISA Kits

General

Antigen Nerve Growth Factor beta (NGFB) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(16), (13), (10), (4), (3), (3), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Supplier Log in to see

Product Details NGFB ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of beta-NGF in human serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of human beta-NGF.
Sensitivity <1 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • human beta-NGF Mix-4x 8 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x 8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details NGFB ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name beta-NGF (NGFB ELISA Kit Abstract)
Background NGF (beta-NGF)is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervoussystem. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons
Molecular Weight 13 kDa
Gene ID 4803
NCBI Accession NP_002497
UniProt P01138
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details NGFB ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-NGF has been pre-coated onto a microplate. Standards, samples and human beta-NGF mix are pipetted into the wells and any beta-NGF present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 5 ng/mL standard. Gently vortex to mix.Take 200 μL beta-NGF standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 2000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 150 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. human beta-NGF Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1492 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:2.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of beta-NGF Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

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Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

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Supplier Images
ELISA image for NGFB ELISA Kit (Nerve Growth Factor beta) (ABIN2506945) Nerve Growth Factor beta (NGFB) ELISA Kit