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|Application / Reactivity||Mouse (Murine)|
|ELISA||10 ELISA Kits|
|Antigen||Nerve Growth Factor beta (NGFB) ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||31.2-2000 pg/mL|
|Minimum Detection Limit||31.2 pg/mL|
|5 references available|
|Supplier||Log in to see|
Product Details NGFB ELISA KitTarget details Application Details Handling References for NGFB Kit (ABIN411336) Images
|Purpose||For quantitative detection of mouse NGF in cell culture supernates and serum.|
|Sample Type||Cell Culture Supernatant, Serum|
|Specificity||Natural and recombinant mouse NGF|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: S122-G241
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|Alternative Name||NGF (NGFB ELISA Kit Abstract)|
|Background||Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.|
|Research Area||Neurology, Growth Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse NGF standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
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|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for NGFB Kit (ABIN411336)Product Details NGFB ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Guo, Sun, Xu et al.: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, 2015 (PubMed).
Fink, Connor, Kelley et al.: "Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution." in: PLoS ONE, Vol. 9, Issue 11, pp. e112737, 2014 (PubMed).
Tan, Xie, Liu et al.: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses Aβ(1-42)-mediated memory deficits in rats." in: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, 2013 (PubMed).
Wang, Zhao, Fan et al.: "Research on the mechanism of Zuogui Pill and Yougui Pill in promoting axonal regeneration in model rats of autoimmune encephalomyelitis." in: Chinese journal of integrative medicine, Vol. 16, Issue 2, pp. 167-72, 2010 (PubMed).
Sanico, Stanisz, Gleeson et al.: "Nerve growth factor expression and release in allergic inflammatory disease of the upper airways." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 5, pp. 1631-5, 2000 (PubMed).
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