Mouse (Murine) NGFB ELISA Kit

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Antigen
Nerve Growth Factor beta (NGFB) ELISA Kits
  • NGFB
  • ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
  • hsan5
  • beta-ngf
  • Ngfb
  • Beta-NGF
  • HSAN5
  • beta-NGF
  • nerve growth factor (beta polypeptide)
  • beta-Nerve growth factor
  • nerve growth factor
  • NGF
  • ngf
  • FPV072
  • FPV076
  • Ngf
Epitope
AA 122-241
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
20
15
11
5
3
2
2
2
1
Method Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Options
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Catalog No. ABIN411336
$ 300.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.70292 ABIN415528 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.70292 ABIN2702851 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 40-10.000 pg/mL 40 pg/mL Log in to see
9.70292 ABIN2708582 Sandwich ELISA Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
9.70292 ABIN2113129 Cell Culture Supernatant, Serum Log in to see
1 ABIN4993006 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.041-10 ng/mL 0.041 ng/mL Log in to see
1 ABIN5594870 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2685071 Sandwich ELISA Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see 3
1 ABIN1112765 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN1847018 Serum, Plasma, Tissue Homogenate, Cell Lysate, Urine, Cell Culture Supernatant, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2116281 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN455963 Log in to see

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General

Antigen Nerve Growth Factor beta (NGFB) ELISA Kits
Epitope AA 122-241
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(20), (15), (11), (5), (3), (2), (2), (2), (1)
Method Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 5 references available
Supplier Log in to see

Product Details NGFB ELISA Kit

Target details Application Details Handling ProductDetails: References for NGFB Kit (ABIN411336) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse NGF/NGF beta
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: S122-G241
Cross-Reactivity (Details) There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
Sensitivity <1pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: S122-G241

Target details

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Antigen
Alternative Name NGF (NGFB ELISA Kit Abstract)
Background

Protein Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. .

Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Synonyms: Beta-nerve growth factor,Beta-NGF,Ngf,Ngfb,

Full Gene Name: Beta-nerve growth factor

Cellular Localisation: Secreted.
Gene ID 18049
UniProt P01139
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the NGF-beta family.

Plate Pre-coated
Protocol mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse NGF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 136, Standard deviation: 5.3, CV(%): 3.9
  • Sample 2: n=16, Mean(pg/ml): 727, Standard deviation: 41.44, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 1326, Standard deviation: 83.54, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 153, Standard deviation: 6.58, CV(%): 4.3
  • Sample 2: n=24, Mean(pg/ml): 839, Standard deviation: 53.7, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 1645, Standard deviation: 121.73, CV(%): 7.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for NGFB Kit (ABIN411336)

Product Details NGFB ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, 2015

Fink, Connor, Kelley, Steele, Hollingsworth, Tempero: "Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution." in: PLoS ONE, Vol. 9, Issue 11, pp. e112737, 2014

Tan, Xie, Liu, Chen, Zheng, Tong, Tian: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses A?(1-42)-mediated memory deficits in rats." in: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, 2013

Wang, Zhao, Fan, Gong, Li, Qi, Liu: "Research on the mechanism of Zuogui Pill and Yougui Pill in promoting axonal regeneration in model rats of autoimmune encephalomyelitis." in: Chinese journal of integrative medicine, Vol. 16, Issue 2, pp. 167-72, 2010

Background publications

Sanico, Stanisz, Gleeson, Bora, Proud, Bienenstock, Koliatsos, Togias: "Nerve growth factor expression and release in allergic inflammatory disease of the upper airways." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 5, pp. 1631-5, 2000

Images

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Supplier Images
ELISA image for NGFB ELISA Kit (Nerve Growth Factor beta) (ABIN411336) Mouse NGF/NGF beta PicoKine ELISA Kit standard curve