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anti-Human NGFR antibody for Flow Cytometry

Recommended NGFR Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor Receptor (NGFR) Antibodies
  • ngfr
  • wu:fb38c02
  • wu:fc10h01
  • CD271
  • Gp80-LNGFR
  • TNFRSF16
  • p75(NTR)
  • p75NTR
  • LNGFR
  • RNNGFRR
  • Tnfrsf16
  • p75
  • NRH1b
  • fullback
  • nrh1
  • p75ntr
  • tnfrsf16
  • p75NGFR
  • nerve growth factor receptor
  • neurotrophin receptor associated death domain
  • nerve growth factor receptor (TNFR superfamily, member 16)
  • NGFR
  • nradd
  • Ngfr
  • ngfr
Reactivity
Human
260
111
101
28
27
26
23
12
10
10
9
6
5
4
3
3
2
2
1
1
Host
Mouse
135
114
35
5
1
Clonality
Monoclonal
Conjugate
This NGFR antibody is un-conjugated
17
15
15
13
5
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
199
107
102
82
59
48
40
31
16
14
13
6
6
6
5
4
2
1
1
1
Options
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Catalog No. ABIN969315
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.149391 ABIN2658564 FACS ICFC APC Mouse IgG1 kappa Log in to see ME20-4 8
13.149391 ABIN2661630 ICC FACS IF ICFC IP IHC WB FITC Mouse IgG1 kappa Log in to see ME20-4 9
13.149391 ABIN2663264 FACS ICFC PE Mouse IgG1 kappa Log in to see ME20-4 7
13.149391 ABIN2660065 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see ME20-4 7
13.149391 ABIN2664471 FACS Mouse IgG1 kappa Log in to see ME20-4 7
13.149391 ABIN4897219 FACS Biotin Mouse IgG1 AA 29-250 Log in to see 74902
13.149391 ABIN4897218 FACS Allophycocyanin Mouse IgG1 AA 29-250 Log in to see 74902
13.149391 ABIN4897221 FACS PE Mouse IgG1 AA 29-250 Log in to see 74902
13.149391 ABIN4897220 FACS Alexa Fluor 700 Mouse IgG1 AA 29-250 Log in to see 74902
13.149391 ABIN1844512 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see
1 ABIN951261 IHC (fro) FACS IF IHC (p) IP Neut WB Biotin Mouse IgG1 Log in to see ME20-4 2
1 ABIN951262 IHC (fro) FACS IF IHC (p) IP Neut WB FITC Mouse IgG1 Log in to see ME20-4 2
1 ABIN951264 FACS PE Mouse IgG1 Log in to see ME20-4 2
1 ABIN951263 IHC (fro) FACS IF IHC (p) IP Neut WB Mouse IgG1 Log in to see ME20-4 1
1 ABIN951260 FACS APC Mouse IgG1 Log in to see ME20-4 2
1 ABIN725660 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 287-337 Log in to see Polyclonal 3
1 ABIN2869468 ELISA FACS ICC WB Mouse IgG1 Log in to see 2F1C2 4
1 ABIN180341 FACS IF IHC (p) IP WB Mouse IgG1 AA 1-160, N-Term Log in to see NGFR5
1 ABIN187476 FACS IP Rat IgG2a Log in to see Nagy-1
1 ABIN187600 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see NGFR5

Top referenced anti-NGFR antibody for Flow Cytometry

Similar anti-NGFR Antibodies

Application / Reactivity Human
Confocal Microscopy (CM) 2 Antibodies
ELISA 91 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 99 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 52 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 71 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 39 Antibodies

General

Antigen Nerve Growth Factor Receptor (NGFR) Antibodies
Reactivity Human
(260), (111), (101), (28), (27), (26), (23), (12), (10), (10), (9), (6), (5), (4), (3), (3), (2), (2), (1), (1)
Host Mouse
(135), (114), (35), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NGFR antibody is un-conjugated
(17), (15), (15), (13), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(199), (107), (102), (82), (59), (48), (40), (31), (16), (14), (13), (6), (6), (6), (5), (4), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-NGFR Antibody

Target Details NGFR Application Details Handling References for anti-NGFR Antibody (ABIN969315) Images
Purification Ascites
Immunogen Purified recombinant fragment of human NGFR expressed in E. coli.
Clone 2F1C2
Isotype IgG1

Target Details NGFR

Product Details anti-NGFR Antibody Application Details Handling References for anti-NGFR Antibody (ABIN969315) Images back to top
Antigen
Alternative Name NGFR (NGFR Antibody Abstract)
Background Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).
Molecular Weight 45 kDa
Gene ID 4804
HGNC 4804
Research Area Growth Factors, Receptors
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis

Application Details

Product Details anti-NGFR Antibody Target Details NGFR Handling References for anti-NGFR Antibody (ABIN969315) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-NGFR Antibody Target Details NGFR Application Details References for anti-NGFR Antibody (ABIN969315) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-NGFR Antibody (ABIN969315)

Product Details anti-NGFR Antibody Target Details NGFR Application Details Handling Images back to top
Product cited in:

He, Garcia: "Structure of nerve growth factor complexed with the shared neurotrophin receptor p75." in: Science (New York, N.Y.), Vol. 304, Issue 5672, pp. 870-5, 2004

Hu, Zhang, Qin, Xu, Swaab, Zhou: "Increased p75(NTR) expression in hippocampal neurons containing hyperphosphorylated tau in Alzheimer patients." in: Experimental neurology, Vol. 178, Issue 1, pp. 104-11, 2002

Background publications

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Fantini, Johansson: "Expression of growth-associated protein 43 and nerve growth factor receptor in human skin: a comparative immunohistochemical investigation." in: The Journal of investigative dermatology, Vol. 99, Issue 6, pp. 734-42, 1993

Images

Product Details anti-NGFR Antibody Target Details NGFR Application Details Handling References for anti-NGFR Antibody (ABIN969315) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-NGFR antibody (Nerve Growth Factor Receptor) (ABIN969315) Figure 1: Flow cytometric analysis of EC cells using NGFR mouse mAb (green) and negat...
Immunocytochemistry (ICC) image for anti-NGFR antibody (Nerve Growth Factor Receptor) (ABIN969315) Figure 2:Immunofluorescence analysis of EC cells using NGFR mouse mAb (green) . Red: ...
Western Blotting (WB) image for anti-NGFR antibody (Nerve Growth Factor Receptor) (ABIN969315) Figure 3: Western blot analysis using NGFR mouse mAb against NGFR-hIgGFc transfected ...