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Human NGFR ELISA Kit

Recommended NGFR Product (supplied by: Log in to see )

Antigen
Nerve Growth Factor Receptor (NGFR) ELISA Kits
  • CD271
  • fullback
  • Gp80-LNGFR
  • LNGFR
  • ngfr
  • nrh1
  • NRH1b
  • p75
  • p75(NTR)
  • p75NGFR
  • p75NTR
  • p75ntr
  • RNNGFRR
  • tnfrsf16
  • Tnfrsf16
  • TNFRSF16
  • wu:fb38c02
  • wu:fc10h01
Epitope
AA 29-250
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
4
3
3
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN2859309
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012207823 ABIN857881 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012207823 ABIN625340 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 80 pg/mL-20 ng/mL 80 pg/mL Log in to see
0.0012207823 ABIN1164389 Log in to see
0.0012207823 ABIN2109998 Sandwich ELISA Log in to see

Similar NGFR ELISA Kits

Application / Reactivity Human
ELISA 5 ELISA Kits

General

Antigen Nerve Growth Factor Receptor (NGFR) ELISA Kits
Epitope AA 29-250
Reactivity Human
Kits with alternative reactivity to:
(4), (3), (3), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Supplier Log in to see

Product Details NGFR ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of human TNFRSF16 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human TNFRSF16
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human TNFRSF16 antibod - 1
  • Lyophilized recombinant human TNFRSF16 standar - 10ng/tubex2
  • Biotinylated anti- human TNFRSF16 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: sf21
Immunogen sequence: K29-N250

Target details

Product Details NGFR ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name NGFR (NGFR ELISA Kit Abstract)
Background Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
Gene ID 4804
UniProt P08138
Research Area Growth Factors, Receptors
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details NGFR ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 - N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 81, Standard deviation: 4.21, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 330, Standard deviation: 21.45, CV(%): 6.5
  • Sample 3: n=16, Mean(pg/ml): 1560, Standard deviation: 90.48, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 85, Standard deviation: 5.27, CV(%): 6.2
  • Sample 2: n=24, Mean(pg/ml): 360, Standard deviation: 27, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 1350, Standard deviation: 90.45, CV(%): 6.7
Restrictions For Research Use only

Handling

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Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details NGFR ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for NGFR ELISA Kit (Nerve Growth Factor Receptor) (ABIN2859309) Human TNFRSF16/NGFR PicoKine ELISA Kit standard curve