anti-Human p65 antibody for Electrophoretic Mobility-Shift Assay

Recommended p65 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antibodies
  • NFKB3
  • p65
  • NFkB
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitope
N-Term, NLS Domain
129
84
67
63
49
44
40
38
38
37
26
26
25
18
17
16
15
15
13
13
13
12
11
10
9
8
7
6
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1137
653
506
72
56
55
30
25
25
23
13
8
8
6
4
2
1
1
Host
Rabbit
994
130
17
11
1
1
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
30
28
22
20
18
17
15
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
832
429
325
260
198
163
105
90
88
28
18
18
15
12
12
6
6
6
5
4
4
2
2
2
1
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Catalog No. ABIN5556664
$ 636.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5556626 EMSA EIA IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN121827 EMSA EIA IP WB Sheep AA 12-317 Log in to see Polyclonal
1 ABIN121887 EMSA EIA IHC (fro) IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN117925 EMSA EIA IP WB Rabbit N-Term Log in to see Polyclonal

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antibodies
Epitope N-Term, NLS Domain
(129), (84), (67), (63), (49), (44), (40), (38), (38), (37), (26), (26), (25), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1137), (653), (506), (72), (56), (55), (30), (25), (25), (23), (13), (8), (8), (6), (4), (2), (1), (1)
Host Rabbit
(994), (130), (17), (11), (1), (1)
Clonality
Polyclonal
Conjugate This p65 antibody is un-conjugated
(30), (28), (22), (20), (18), (17), (15), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(832), (429), (325), (260), (198), (163), (105), (90), (88), (28), (18), (18), (15), (12), (12), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1)
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Product Details anti-p65 Antibody

Target Details p65 Application Details Handling Images
Sequence Peptide Sequence: R-I-E-E-K-R-K-R-T-Y-E-T
Specificity This affinity purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFκB heterodimer. Therefore, the antibody selectively binds to the activated form of NFκB. Anti-NFκB p65 NLS may react non-specifically with other proteins.
Characteristics Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Purification Affinity Purified
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkappaB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details p65

Product Details anti-p65 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
Background NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. IκB- α binding masks the NFκB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFκB and set off signalling cascades that ultimately converge on the IκB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IκB-α and this phophorylation event targets IκB-α for degradation. As a consequence, NFκB NLS is uncovered and nuclear translocation occurs.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
Gene ID 5970
NCBI Accession NP_001138610
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-p65 Antibody Target Details p65 Handling Images back to top
Application Notes Suitable for Immunohistochemistry, Immunoblotting, ELISA and supershift assays. Thisproduct was assayed by Immunoblot and found to be reactive against NFκB p65 NLS at adilution of 1: 2,000 followed by reaction with Peroxidase conjugated Affinity Purified
Restrictions For Research Use only

Handling

Product Details anti-p65 Antibody Target Details p65 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.