You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PKC zeta antibody for Immunohistochemistry (Frozen Sections)

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • 14-3-3-zetaisoform
  • AI098070
  • aPKCzeta
  • C80388
  • pkc-zeta
  • PKC-ZETA
  • pkc2
  • PKC2
  • pkcz
  • Pkcz
  • PKCzeta
  • PRKCZ
  • prkcz-A
  • r14-3-3
  • R74924
  • si:ch211-150o23.4
  • TPKC
  • zetaPKC
Epitope
AA 404-591
46
38
17
14
11
7
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
161
94
78
5
4
2
2
2
1
1
Host
Rabbit
189
4
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
9
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
128
91
90
31
27
11
10
10
4
3
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1860239
$ 365.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088254584 ABIN4345864 ELISA IHC IHC (fro) IHC (p) IP WB Rabbit IgG Log in to see R14-K

Similar anti-PKC zeta Antibodies

Application / Reactivity Human
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 90 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 102 Antibodies

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope AA 404-591
(46), (38), (17), (14), (11), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(161), (94), (78), (5), (4), (2), (2), (2), (1), (1)
Host Rabbit
(189), (4), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(9), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(128), (91), (90), (31), (27), (11), (10), (10), (4), (3), (2), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP FDIITDNPDM NTEDYLFQVI LEKPIRIPRF LSVKASHVLK GFLNKDPKER LGCRPQTGFS DIKSHAFFRS IDWDLLEKKQ ALPPFQPQIT DDYGLDNFDT QFTSEPVQLT PDDEDAIKRI DQSEFEGFEY INPLLLSTEE S
Specificity It has been selected for its ability to recognize PKCz in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen PKCz (AA 404-591)
Isotype IgG

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling Images back to top
Antigen
Alternative Name Protein Kinase C Zeta (PKCz) (PRKCZ Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PKCz (Pro404~Ser591) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (AA 404-591) (ABIN1860239) anti-Protein Kinase C, zeta (PRKCZ) (AA 404-591) antibody