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anti-Human PKC zeta antibody for Western Blotting

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • 14-3-3-zetaisoform
  • AI098070
  • aPKCzeta
  • C80388
  • pkc-zeta
  • PKC-ZETA
  • pkc2
  • PKC2
  • pkcz
  • Pkcz
  • PKCzeta
  • PRKCZ
  • prkcz-A
  • r14-3-3
  • R74924
  • si:ch211-150o23.4
  • TPKC
  • zetaPKC
Epitope
N-Term
45
38
17
14
11
7
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
157
91
75
4
4
2
2
2
Host
Rabbit
185
4
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
8
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
125
91
87
30
25
11
10
9
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2775614
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN391018 IHC (p) WB Rabbit Ig AA 166-197, N-Term Log in to see Polyclonal 1
0.12303223 ABIN1531510 IHC ELISA WB Rabbit IgG pThr560 Log in to see Polyclonal
0.12303223 ABIN1531192 IF IHC ELISA WB Rabbit IgG pThr410 Log in to see Polyclonal
0.12303223 ABIN1532208 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1532525 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN256888 ICC IF IHC (p) IHC WB Rabbit IgG Thr410 Log in to see EP1491Y
0.12303223 ABIN2705133 WB Rabbit C-Term, pSer560 Log in to see Polyclonal
0.12303223 ABIN2705134 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN258979 ICC IF IHC (p) IHC WB Rabbit IgG Thr560 Log in to see EP2037AY
0.12303223 ABIN2462644 ELISA WB Rabbit Log in to see Polyclonal
0.12303223 ABIN631227 WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN1874308 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2499430 IF IHC ELISA WB Rabbit IgG Thr560 Log in to see Polyclonal
0.12303223 ABIN2499429 IHC ELISA WB Rabbit IgG pThr560 Log in to see Polyclonal
0.12303223 ABIN2499432 IF IHC ELISA WB Rabbit IgG pThr410 Log in to see Polyclonal
0.12303223 ABIN2499431 IF IHC ELISA WB Rabbit IgG Thr410 Log in to see Polyclonal
0.12303223 ABIN2495508 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.12303223 ABIN3182129 ELISA IHC WB Rabbit IgG pThr560 Log in to see Polyclonal
0.12303223 ABIN3182128 ELISA IF IHC WB Rabbit IgG pThr410 Log in to see Polyclonal
0.12303223 ABIN1860239 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 404-591 Log in to see Polyclonal

Top referenced anti-PKC zeta antibody for Western Blotting

Similar anti-PKC zeta Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Cow (Bovine) Dog (Canine) Rabbit
Western Blotting (WB) 99 Antibodies 49 Antibodies 60 Antibodies 1 Antibodies 1 Antibodies 3 Antibodies
RNA Interference (RNAi) 2 Antibodies
Radioimmunoassay (RIA) 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 7 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 8 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 87 Antibodies 44 Antibodies

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope N-Term
(45), (38), (17), (14), (11), (7), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(157), (91), (75), (4), (4), (2), (2), (2)
Host Rabbit
(185), (4), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(8), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(125), (91), (87), (30), (25), (11), (10), (9), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling References for anti-PKC zeta Antibody (ABIN2775614) Images
Blocking Peptide Blocking peptide for this product available: ABIN982222
Sequence MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP
Predicted Reactivity Human : 100 %, Mouse : 90 %, Rat : 90 %, Bovine : 70 %, Dog : 70 %, Guinea pig : 70 %, Horse : 70 %
Characteristics This is a rabbit polyclonal antibody against PRKCZ. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRKCZ

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling References for anti-PKC zeta Antibody (ABIN2775614) Images back to top
Antigen
Alternative Name PRKCZ (PRKCZ Antibody Abstract)
Background Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Molecular Weight 45 kDa
Gene ID 5590
NCBI Accession NM_001033581, NP_001028753
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling References for anti-PKC zeta Antibody (ABIN2775614) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 409 AA

Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details References for anti-PKC zeta Antibody (ABIN2775614) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PKC zeta Antibody (ABIN2775614)

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling Images back to top
Background publications

Xie, Dong, Zhang, Cui, Cohen, Riek, Neumann, Schlattner, Zou: "Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6366-75, 2006

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling References for anti-PKC zeta Antibody (ABIN2775614) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (N-Term) (ABIN2775614) WB Suggested Anti-PRKCZ Antibody Titration: 0.2-1 ug/ml
Positive Control...