anti-Human PKC zeta antibody for Western Blotting

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • TPKC
  • PKCzeta
  • prkcz-A
  • pkc-zeta
  • PRKCZ
  • pkc2
  • PKC-ZETA
  • PKC2
  • AI098070
  • C80388
  • Pkcz
  • R74924
  • aPKCzeta
  • zetaPKC
  • 14-3-3-zetaisoform
  • r14-3-3
  • pkcz
  • si:ch211-150o23.4
  • protein kinase C, zeta
  • prkcz
  • PRKCZ
  • Prkcz
Epitope
N-Term
40
31
17
14
12
6
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
160
108
91
8
7
6
3
3
3
2
2
2
2
2
1
Host
Rabbit
186
4
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
9
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
136
91
86
35
29
14
13
7
6
3
3
2
2
2
1
1
Options
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Catalog No. ABIN2775614
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1581392 ABIN391018 IHC (p) WB Rabbit Ig AA 166-197, N-Term Log in to see Polyclonal 1
3.1581392 ABIN4345863 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.1581392 ABIN1531192 IF IHC ELISA WB Rabbit IgG pThr410 Log in to see Polyclonal
3.1581392 ABIN1531510 IHC ELISA WB Rabbit IgG pThr560 Log in to see Polyclonal
3.1581392 ABIN3182129 ELISA IHC WB Rabbit IgG pThr560 Log in to see Polyclonal
3.1581392 ABIN3182128 ELISA IF IHC WB Rabbit IgG pThr410 Log in to see Polyclonal
3.1581392 ABIN3186477 ELISA IF IHC WB Rabbit IgG Thr33 Log in to see Polyclonal
3.1581392 ABIN3186476 ELISA IF IHC WB Rabbit IgG Thr322 Log in to see Polyclonal
3.1581392 ABIN1532525 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN1532208 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN2705133 WB Rabbit C-Term, pSer560 Log in to see Polyclonal
3.1581392 ABIN2705134 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal
3.1581392 ABIN2462644 ELISA WB Rabbit Log in to see Polyclonal
3.1581392 ABIN1874308 IHC WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN4345865 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN1860239 ICC IHC IP WB Rabbit IgG AA 404-591 Log in to see Polyclonal
3.1581392 ABIN631227 WB Rabbit N-Term Log in to see Polyclonal
3.1581392 ABIN2564714 IHC WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN519207 WB Mouse AA 1-592, full length Log in to see Polyclonal
3.1581392 ABIN562397 RNAi ELISA WB Mouse IgG2b kappa AA 165-255, partial Log in to see 2D1

Top referenced anti-PKC zeta antibody for Western Blotting

Similar anti-PKC zeta Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Rabbit Cow (Bovine) Dog (Canine) Guinea Pig Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 5 Antibodies 4 Antibodies 1 Antibodies
Western Blotting (WB) 107 Antibodies 62 Antibodies 77 Antibodies 2 Antibodies 2 Antibodies 6 Antibodies 2 Antibodies 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Radioimmunoassay (RIA) 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 8 Antibodies 9 Antibodies

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope N-Term
(40), (31), (17), (14), (12), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(160), (108), (91), (8), (7), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(186), (4), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(9), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(136), (91), (86), (35), (29), (14), (13), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling ProductDetails: References for anti-PKC zeta antibody (ABIN2775614) Images
Blocking Peptide Blocking peptide for this product available: ABIN982222
Sequence MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP
Predicted Reactivity Cow: 77%, Dog: 75%, Guinea Pig: 75%, Horse: 75%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against PRKCZ. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRKCZ

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling ProductDetails: References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Antigen
Alternative Name PRKCZ (PRKCZ Antibody Abstract)
Background Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PKC2, PKC-ZETA
Protein Interaction Partner: MAPK7, PRKCZ, AKT2, AKT1, Sqstm1, PARD6A, NUMB, NPM1, HIST1H1B, CFL1, C1QBP, HSP90AA1, NCF1, MARCKS, PPP1R14A, HIST1H1A, UBC, VHL, LRRK2, AJUBA, PARD6B, PAWR, Smurf1, Smurf2, Pard3, HIST3H3, CD44, NCOA3, TFF1, RELA, ESR1, MBP, TRIM41, PIAS4, CCDC115, ZNF7
Protein Size: 409
Molecular Weight 45 kDa
Gene ID 5590
NCBI Accession NM_001033581, NP_001028753
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling ProductDetails: References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 409 AA

Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details ProductDetails: References for anti-PKC zeta antibody (ABIN2775614) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PKC zeta antibody (ABIN2775614)

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling Images back to top
Background publications

Xie, Dong, Zhang, Cui, Cohen, Riek, Neumann, Schlattner, Zou: "Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6366-75, 2006

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling ProductDetails: References for anti-PKC zeta antibody (ABIN2775614) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (N-Term) (ABIN2775614) WB Suggested Anti-PRKCZ Antibody Titration: 0.2-1 ug/ml
Positive Control...