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anti-Mouse (Murine) PKC zeta antibody for Immunohistochemistry (Frozen Sections)

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • 14-3-3-zetaisoform
  • AI098070
  • aPKCzeta
  • C80388
  • pkc-zeta
  • PKC-ZETA
  • pkc2
  • PKC2
  • pkcz
  • Pkcz
  • PKCzeta
  • PRKCZ
  • prkcz-A
  • r14-3-3
  • R74924
  • si:ch211-150o23.4
  • TPKC
  • zetaPKC
Epitope
AA 254-484
46
38
17
14
11
7
6
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
158
91
76
5
4
2
2
2
1
1
Host
Rabbit
185
4
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
8
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
125
90
87
30
24
11
9
9
3
3
2
2
1
1
1
1
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Catalog No. ABIN1860238
$ 376.20
Plus shipping costs $45.00

Similar anti-PKC zeta Antibodies

Application / Reactivity Mouse (Murine)
ELISA 39 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope AA 254-484
(46), (38), (17), (14), (11), (7), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(158), (91), (76), (5), (4), (2), (2), (2), (1), (1)
Host Rabbit
(185), (4), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(8), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(125), (90), (87), (30), (24), (11), (9), (9), (3), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP FDIITDNPDM NTEDYLFQVI LEKPIRIPRF LSVK
Specificity It has been selected for its ability to recognize PKCz in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen PKCz (AA 254-484)
Isotype IgG

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling Images back to top
Antigen
Alternative Name Protein Kinase C Zeta (PKCz) (PRKCZ Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PKCz (Leu254~Lys484) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (AA 254-484) (ABIN1860238) anti-Protein Kinase C, zeta (PRKCZ) (AA 254-484) antibody