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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Western Blotting (WB)||99 Antibodies||49 Antibodies||60 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies||8 Antibodies||7 Antibodies|
|Immunohistochemistry (IHC)||87 Antibodies||44 Antibodies||44 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||1 Antibodies||1 Antibodies|
|Antigen||Protein Kinase C, zeta (PRKCZ) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PKC zeta antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PKC zeta AntibodyTarget Details PKC zeta Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PKC ζ protein. (region surrounding Pro404)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details PKC zetaProduct Details anti-PKC zeta Antibody Application Details Handling Images back to top
|Alternative Name||PRKCZ (PRKCZ Antibody Abstract)|
|Background||Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activitiesSynonyms: PKC zeta, PKC-zeta, PKC2, Protein kinase C zeta type, nPKC-zeta|
|Molecular Weight||approx. 70 to 85 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling|
Application DetailsProduct Details anti-PKC zeta Antibody Target Details PKC zeta Handling Images back to top
ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling back to top