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anti-Cow (Bovine) PRKACA antibody for Immunoprecipitation

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • Calpha
  • Cs-PKA
  • PKA
  • PKACA
  • Pkaca
  • PKCA1
  • PKCD
  • PRKAR1
Epitope
Catalytic Subunit
18
12
8
7
5
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
94
28
21
1
Host
Rabbit
66
28
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
80
29
16
11
11
11
6
5
4
2
2
1
1
1
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Catalog No. ABIN127012
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008941957 ABIN4345784 IP WB Rabbit Log in to see Polyclonal

Similar anti-PRKACA Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Pig (Porcine) Rat (Rattus) Sheep (Ovine)
Western Blotting (WB) 2 Antibodies 83 Antibodies 19 Antibodies 1 Antibodies 13 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies 6 Antibodies 3 Antibodies 1 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 8 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 5 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope Catalytic Subunit
(18), (12), (8), (7), (5), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(94), (28), (21), (1)
Host Rabbit
(66), (28), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(80), (29), (16), (11), (11), (11), (6), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Specificity Recognizes a 40 kDa PKA on SDS-PAGE immunoblots
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 11 of the catalytic subunit of PKA alpha isoform.

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKACA (PRKACA Antibody Abstract)
Background Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Gene ID 5566
NCBI Accession NP_002721
UniProt P17612
Research Area Cancer, Kinases/Phosphatases
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes Western blot: 0.5-4 μg/mL, Jukat cell lysate can be used as a positive control. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top
Supplier Images
 image for anti-PRKACA antibody (Protein Kinase A, alpha) (Catalytic Subunit) (ABIN127012) anti-Protein Kinase A, alpha (PRKACA) (Catalytic Subunit) antibody