anti-Human PRKACA antibody for Enzyme Immunoassay

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Epitope
N-Term
20
15
12
7
7
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
111
30
24
4
3
2
2
2
2
2
2
Host
Rabbit
62
43
6
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
95
42
15
13
12
11
5
5
3
2
1
1
1
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Catalog No. ABIN453298
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359056 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope N-Term
(20), (15), (12), (7), (7), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(111), (30), (24), (4), (3), (2), (2), (2), (2), (2), (2)
Host Rabbit
(62), (43), (6), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(95), (42), (15), (13), (12), (11), (5), (5), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling ProductDetails: References for anti-PRKACA antibody (ABIN453298) Images
Specificity This antibody reacts to PRKACA.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the N-terminal(K282) region of human PRKACA

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling ProductDetails: References for anti-PRKACA antibody (ABIN453298) Images back to top
Antigen
Alternative Name PRKACA (PRKACA Antibody Abstract)
Background PRKACA phosphorylates a large number of substrates in the cytoplasm and the nucleus.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Gene ID 5566
NCBI Accession NP_002721
UniProt P17612
Research Area Cancer, Kinases/Phosphatases
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling ProductDetails: References for anti-PRKACA antibody (ABIN453298) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details ProductDetails: References for anti-PRKACA antibody (ABIN453298) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-PRKACA antibody (ABIN453298)

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling Images back to top
Background publications

Sugiyama, Chen, Hunter, Taffs, Sitkovsky: "The dual role of the cAMP-dependent protein kinase C alpha subunit in T-cell receptor-triggered T-lymphocytes effector functions." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25256-63, 1993

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling ProductDetails: References for anti-PRKACA antibody (ABIN453298) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (N-Term) antibody (ABIN453298) Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (...