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anti-Human PRKACA antibody for Immunofluorescence

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • Calpha
  • Cs-PKA
  • PKA
  • PKACA
  • Pkaca
  • PKCA1
  • PKCD
  • PRKAR1
Epitope
Center
18
12
8
7
5
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
96
29
23
2
1
1
Host
Rabbit
67
29
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
82
29
17
11
11
11
6
6
4
2
2
1
1
1
Supplier
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Catalog No. ABIN2855997
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN2499391 IF IHC ELISA WB Rabbit IgG Thr197 Log in to see Polyclonal
0.32040194 ABIN2499392 IF IHC ELISA WB Rabbit IgG pThr197 Log in to see Polyclonal
0.32040194 ABIN2856513 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.32040194 ABIN2495468 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN317665 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN1885909 IF WB Rabbit AA 1-340 Log in to see Polyclonal
0.0010840179 ABIN408069 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal 1
0.0010840179 ABIN2340986 IF IHC ELISA WB Mouse IgG1 kappa AA 563-672 Log in to see 2F11
0.0010840179 ABIN2800195 IF (cc) IF WB Rabbit IgG AA 1-351 Log in to see Polyclonal
0.0010840179 ABIN4218851 IF Rabbit pThr197 Log in to see Polyclonal
0.0010840179 ABIN4345788 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-PRKACA antibody for Immunofluorescence

  • Human Polyclonal PRKACA Primary Antibody for ICC, IF - ABIN408069 (1 Publications): Enns, Morton, Mangalindan, McKnight, Schwartz, Kaeberlein, Kennedy, Rabinovitch, Ladiges: Attenuation of age-related metabolic dysfunction in mice with a targeted disruption of the Cbeta subunit of protein kinase A. in The journals of gerontology. Series A, Biological sciences and medical sciences 2009 (PubMed)

Similar anti-PRKACA Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 7 Antibodies
Immunofluorescence (IF) 9 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 19 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope Center
(18), (12), (8), (7), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(96), (29), (23), (2), (1), (1)
Host Rabbit
(67), (29), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(82), (29), (17), (11), (11), (11), (6), (6), (4), (2), (2), (1), (1), (1)
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Cross-Reactivity Rat (Rattus), Dog (Canine), Sheep (Ovine), Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.
Isotype IgG

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKA C alpha (PRKACA Antibody Abstract)
Background CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity)
Molecular Weight 41 kDa
Gene ID 5566
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HeLa , Jurkat , NT2D1 , mouse brain

Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Format Liquid
Concentration 0.38 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PRKACA antibody (Protein Kinase A, alpha) (Center) (ABIN2855997) WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE antibody diluted at...
Western Blotting (WB) image for anti-PRKACA antibody (Protein Kinase A, alpha) (Center) (ABIN2855997) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-PRKACA antibody (Protein Kinase A, alpha) (Center) (ABIN2855997) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PKA ...
Immunofluorescence (IF) image for anti-PRKACA antibody (Protein Kinase A, alpha) (Center) (ABIN2855997) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha, ant...