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anti-Rat (Rattus) PRKACA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • Calpha
  • Cs-PKA
  • PKA
  • PKACA
  • Pkaca
  • PKCA1
  • PKCD
  • PRKAR1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
71
24
17
1
1
1
Host
Rabbit
62
9
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
57
25
15
11
9
9
5
5
4
2
2
1
1
1
Supplier
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Catalog No. ABIN317665
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089488085 ABIN751897 IHC (p) WB HRP Rabbit IgG Regulatory Subunit 1 alpha Log in to see Polyclonal
0.00089488085 ABIN751890 IHC (p) WB Biotin Rabbit IgG Regulatory Subunit 1 alpha Log in to see Polyclonal
0.00089488085 ABIN751888 IF (p) IHC (p) WB Rabbit IgG Regulatory Subunit 1 alpha Log in to see Polyclonal
0.00089488085 ABIN408069 ICC IF IHC (p) IHC WB Rabbit IgG Val191 Log in to see Polyclonal

Similar anti-PRKACA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 3 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 7 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Western Blotting (WB) 9 Antibodies 15 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Dot Blot (DB) 1 Antibodies

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(71), (24), (17), (1), (1), (1)
Host Rabbit
(62), (9), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(57), (25), (15), (11), (9), (9), (5), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Specificity This antibody detects endogenous levels of PKAalpha/beta cat protein.(region surrounding Lys17)
Purification Affinity Chromatography using epitope-specific immunogen.

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKACA (PRKACA Antibody Abstract)
Background The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93 % amino acid sequence similarity), whereas Cgamma displays 83 % and 79 % similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Molecular Weight approx. 38 kDa
Gene ID 5566
NCBI Accession NP_002721
UniProt P17612
Research Area Cancer, Kinases/Phosphatases
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top
Supplier Images
 image for anti-PRKACA antibody (Protein Kinase A, alpha) (ABIN317665) anti-Protein Kinase A, alpha (PRKACA) antibody