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|Antigen||Protein Kinase A, alpha (PRKACA) Antibodies|
|Epitope||Catalytic Subunit Alternatives|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This PRKACA antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-PRKACA AntibodyTarget Details PRKACA Application Details Handling Images
|Specificity||Recognizes a 40 kDa PKA on SDS-PAGE immunoblots|
|Immunogen||Synthetic peptide surrounding amino acid 11 of the catalytic subunit of PKA alpha isoform.|
Target Details PRKACAProduct Details anti-PRKACA Antibody Application Details Handling Images back to top
|Alternative Name||PRKACA (PRKACA Antibody Abstract)|
|Background||Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha|
|Research Area||Cancer, Kinases/Phosphatases|
|Pathways||NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Western blot: 0.5-4 μg/mL, Jukat cell lysate can be used as a positive control. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
|Buffer||PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C/-80 °C|
|Storage Comment||Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.|
ImagesProduct Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top