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anti-Human SQSTM1 antibody for Immunocytochemistry

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • A170
  • MGC79491
  • OSF-6
  • Osi
  • OSIL
  • p60
  • p62
  • p62B
  • PDB3
  • sb:cb621
  • sqstm1
  • SQSTM1
  • STAP
  • zgc:85784
  • ZIP
  • ZIP3
Epitope
Internal Region
45
40
19
19
18
17
17
14
13
13
7
6
5
5
5
5
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
238
61
60
3
2
1
1
1
Host
Rabbit
184
47
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
13
12
12
8
8
8
5
5
5
3
3
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
88
77
55
48
42
36
33
12
11
7
4
3
2
Supplier
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Catalog No. ABIN2854836
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN3044026 ICC IHC (p) WB Rabbit IgG AA 91-110, N-Term Log in to see Polyclonal
0.0009962841 ABIN449247 ICC IF SimWes WB Rabbit Internal Region, AA 400-450 Log in to see Polyclonal 1
0.0009962841 ABIN449245 ICC IF WB Rabbit Internal Region, AA 350-400 Log in to see Polyclonal
0.0009962841 ABIN334048 ICC IHC (fro) IHC (p) WB Guinea Pig IgG C-Term Log in to see Polyclonal
0.0009962841 ABIN2628720 ICC IHC (p) WB Rabbit IgG AA 91-110 Log in to see Polyclonal
0.0009962841 ABIN1920954 ICC IHC ELISA WB PE Rabbit IgG C-Term, AA 324-353 Log in to see Polyclonal
0.0009962841 ABIN1920953 ICC IHC ELISA WB FITC Rabbit IgG C-Term, AA 324-353 Log in to see Polyclonal
0.0009962841 ABIN1920951 ICC IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 324-353 Log in to see Polyclonal
0.0009962841 ABIN1920952 ICC IHC ELISA WB APC Rabbit IgG C-Term, AA 324-353 Log in to see Polyclonal
0.0009962841 ABIN1860638 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 136-400 Log in to see Polyclonal
0.0009962841 ABIN4343161 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 AA 100-400 Log in to see 5H7E2
0.0009962841 ABIN4343162 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 AA 100-400 Log in to see 5H7E2
0.0009962841 ABIN1737788 ICC IF WB DyLight 650 Rabbit IgG Internal Region, AA 300-450 Log in to see Polyclonal
0.0009962841 ABIN1737790 ICC WB HRP Rabbit IgG Internal Region, AA 300-450 Log in to see Polyclonal
0.0009962841 ABIN1737787 ICC WB Biotin Rabbit IgG Internal Region, AA 300-450 Log in to see Polyclonal
0.0009962841 ABIN4343149 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal
0.0009962841 ABIN4343151 ICC IF WB Biotin Rabbit AA 350-400, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4343152 ICC IF WB DyLight 650 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4343153 ICC IF WB DyLight 488 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4343154 ICC IF WB DyLight 550 Rabbit AA 350-400, Internal Region Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Immunocytochemistry

Similar anti-SQSTM1 Antibodies

Application / Reactivity Zebrafish (Danio rerio) Rat (Rattus) Mouse (Murine)
Flow Cytometry (FACS) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 27 Antibodies
Immunofluorescence (IF) 1 Antibodies 29 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Western Blotting (WB) 2 Antibodies 50 Antibodies
ELISA 7 Antibodies 7 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region
(45), (40), (19), (19), (18), (17), (17), (14), (13), (13), (7), (6), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(238), (61), (60), (3), (2), (1), (1), (1)
Host Rabbit
(184), (47), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(13), (12), (12), (8), (8), (8), (5), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(172), (88), (77), (55), (48), (42), (36), (33), (12), (11), (7), (4), (3), (2)
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (93 %)
Characteristics Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
SQSTM1 antibody [N3C1], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1. The exact sequence is proprietary.
Isotype IgG

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Cellular Localization: Cytoplasm , Late endosome , Nucleus
Molecular Weight 48 kDa
Gene ID 8878
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: A549 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 (untreated) , Huh7 ( 3uM Thapsigargin treatment for 12hr) , *HeLa

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot anal...
Immunohistochemistry (IHC) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on h...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot anal...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Whole cell ...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Flow Cytometry (FACS) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometr...