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anti-Human SQSTM1 antibody for Immunofluorescence

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
AA 324-353, C-Term
50
40
23
19
19
18
18
17
17
17
15
14
11
11
8
7
6
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
299
131
98
8
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
243
49
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
16
16
15
10
10
10
7
7
7
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
238
123
82
80
65
62
37
35
20
13
11
4
4
2
2
Supplier
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Catalog No. ABIN4620406
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.318237 ABIN449244 ICC IF WB Rabbit Internal Region, AA 350-400 Log in to see Polyclonal 1
11.318237 ABIN449246 ICC IF SimWes WB Rabbit Internal Region, AA 400-450 Log in to see Polyclonal 1
11.318237 ABIN393579 IF ELISA WB Mouse IgG2a kappa AA 1-441 Log in to see 2C11 1
11.318237 ABIN4343147 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 27
11.318237 ABIN2854836 ICC FACS IF IP IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
11.318237 ABIN563860 IF ELISA WB Mouse IgG2a kappa AA 1-440, full length Log in to see 2C11 76
11.318237 ABIN4236624 FACS ICC IF WB Mouse IgG2b Log in to see 1478
11.318237 ABIN1327673 IF WB Mouse AA 1-440, full length Log in to see Polyclonal
11.318237 ABIN522357 IF WB Mouse AA 1-440, full length Log in to see Polyclonal
11.318237 ABIN5078601 ICC IF WB Rabbit IgG Log in to see Polyclonal
11.318237 ABIN2476572 IF WB Mouse IgG2a Log in to see 2C11
1 ABIN388979 IF IHC (p) WB Rabbit Ig AA 317-346, C-Term Log in to see Polyclonal 22
1 ABIN4343150 ICC IF IHC IHC (p) IP WB Rabbit AA 425-440 Log in to see Polyclonal 3
1 ABIN1499988 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4E2
1 ABIN1499989 FACS IF IHC (p) WB Mouse IgG1 Log in to see 5A2
1 ABIN1499990 FACS IF WB Mouse IgG2a Log in to see 4C8
1 ABIN1499991 FACS IF WB Mouse IgG1 Log in to see 7F6
1 ABIN1499987 FACS IF IHC (p) WB Mouse IgG2a Log in to see 5A1
1 ABIN1499992 FACS IF WB Mouse IgG2a Log in to see 6A7
1 ABIN4343162 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 AA 100-400 Log in to see 5H7E2 1

Top referenced anti-SQSTM1 antibody for Immunofluorescence

Similar anti-SQSTM1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 35 Antibodies
ELISA 80 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 82 Antibodies
Immunofluorescence (IF) 124 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 239 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope AA 324-353, C-Term
(50), (40), (23), (19), (19), (18), (18), (17), (17), (17), (15), (14), (11), (11), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(299), (131), (98), (8), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(243), (49), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(16), (16), (15), (10), (10), (10), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(238), (123), (82), (80), (65), (62), (37), (35), (20), (13), (11), (4), (4), (2), (2)
Pubmed 12 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN4620406) Images
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human SQSTM1
Isotype Ig

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN4620406) Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62
Molecular Weight 47687 Da
Gene ID 8878
NCBI Accession NP_001135770
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN4620406) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN4620406) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SQSTM1 Antibody (ABIN4620406)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Background publications

Sansanwal, Sarwal: "p62/SQSTM1 prominently accumulates in renal proximal tubules in nephropathic cystinosis." in: Pediatric nephrology (Berlin, Germany), Vol. 27, Issue 11, pp. 2137-44, 2012

Soontornniyomkij, Risbrough, Young, Soontornniyomkij, Jeste, Achim: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, 2012

Lee, Shin, Yuk, Shi, Choi, Lee, Huang, Kim, Kim, Lee, Jo: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, 2011

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, 2011

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, 2011

Chu, Wang, Wu, Lei: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, 2010

Lin, Whang, Donner, Du, Lorch, He, Jiang, Price, Moore, Ruan: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, 2010

Maynard, Dvorak, Khailova, Dobrenen, Arganbright, Halpern, Kurundkar, Maheshwari, Dvorak: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, 2010

Zhang, Lowry, Guarente, Haimovich: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, 2010

Tal, Sasai, Lee, Yordy, Shadel, Iwasaki: "Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 8, pp. 2770-5, 2009

Kobayashi, Volden, Timm, Mao, Xu, Liang: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, 2009

Ju, Miller, Hanson, Weihl: "Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease." in: The Journal of biological chemistry, Vol. 283, Issue 44, pp. 30289-99, 2008

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN4620406) back to top
Supplier Images
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 324-353) (ABIN4620406) Confocal immunofluorescent analysis of SQSTM1 Antibody  with Hela cell followed by Al...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 324-353) (ABIN4620406) Immunofluorescence staining of Autophagy SQSTM1 Antibody  on Methanol-fixed and PFA f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 324-353) (ABIN4620406) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SQSTM1 Anti...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 324-353) (ABIN4620406) Western blot analysis of SQSTM1 (arrow) using SQSTM1 Antibody . 293 cell lysates (2 u...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 324-353) (ABIN4620406) Western blot analysis of SQSTM1 Antibody  pre-incubated without (Lane 1) and with (La...