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|Application / Reactivity||Human|
|Dot Blot (DB)||36 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||31 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||29 Antibodies|
|Immunofluorescence (IF)||62 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||144 Antibodies|
|Antigen||Sequestosome 1 (SQSTM1) Antibodies|
|Epitope||AA 324-353, C-Term Alternatives|
|Conjugate||This SQSTM1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|12 references available|
|Supplier||Log in to see|
Product Details anti-SQSTM1 AntibodyTarget Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN1449619) Images
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human SQSTM1|
Target Details SQSTM1Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN1449619) Images back to top
|Alternative Name||SQSTM1 (SQSTM1 Antibody Abstract)|
|Background||SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62|
|Molecular Weight||47687 Da|
|Pathways||NF-kappaB Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN1449619) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN1449619) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-SQSTM1 Antibody (ABIN1449619)Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Sansanwal, Sarwal: "p62/SQSTM1 prominently accumulates in renal proximal tubules in nephropathic cystinosis." in: Pediatric nephrology (Berlin, Germany), Vol. 27, Issue 11, pp. 2137-44, 2012 (PubMed).
Okamoto, Sakimoto, Imai et al.: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, 2011 (PubMed).
Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira et al.: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, 2011 (PubMed).
Soontornniyomkij, Risbrough, Young et al.: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, 2012 (PubMed).
Lee, Shin, Yuk et al.: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, 2011 (PubMed).
Zhang, Lowry, Guarente et al.: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, 2010 (PubMed).
Lin, Whang, Donner et al.: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, 2010 (PubMed).
Chu, Wang, Wu et al.: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, 2010 (PubMed).
Maynard, Dvorak, Khailova et al.: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, 2010 (PubMed).
Kobayashi, Volden, Timm et al.: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, 2009 (PubMed).
Tal, Sasai, Lee et al.: "Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 8, pp. 2770-5, 2009 (PubMed).
Ju, Miller, Hanson et al.: "Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease." in: The Journal of biological chemistry, Vol. 283, Issue 44, pp. 30289-99, 2008 (PubMed).
ImagesProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN1449619) back to top