anti-Human SQSTM1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
AA 300-440
52
40
22
19
19
18
18
18
17
17
15
14
12
10
8
8
6
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
331
151
100
8
5
5
5
4
4
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
256
68
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
18
17
17
11
10
10
8
8
8
6
6
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
264
137
95
85
79
64
52
35
32
14
13
4
3
3
2
Supplier
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Catalog No. ABIN4343147
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN113450 IHC (fro) IHC (p) WB Guinea Pig C-Term Log in to see Polyclonal 3
1 ABIN334048 ICC IHC (fro) IHC (p) WB Guinea Pig IgG AA 421-440 Log in to see Polyclonal
1 ABIN113451 IHC (fro) IHC (p) WB Guinea Pig N-Term Log in to see Polyclonal 3
1 ABIN350826 IHC (fro) IHC (p) WB Sheep AA 300-360 Log in to see Polyclonal 10
1 ABIN350827 IHC (fro) IHC (p) WB Rabbit AA 300-360 Log in to see Polyclonal 10
1 ABIN350828 IHC (fro) IHC (p) WB Rabbit IgG AA 300-360 Log in to see Polyclonal 10
1 ABIN350829 IHC (fro) IHC (p) WB Sheep IgG AA 300-360 Log in to see Polyclonal 10
1 ABIN4343146 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962912 ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962906 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962907 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962910 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962914 ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962911 ICC IF IHC (fro) IHC (p) WB DyLight 650 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962909 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962908 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962913 IHC (fro) IHC (p) WB HRP Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN5627955 FACS ICC IF IHC IHC (fro) WB DyLight 650 Mouse IgG2b Log in to see 1478
1 ABIN5627948 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 700 Mouse IgG2b Log in to see 1478
1 ABIN5627954 FACS ICC IF IHC IHC (fro) WB DyLight 550 Mouse IgG2b Log in to see 1478

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope AA 300-440
(52), (40), (22), (19), (19), (18), (18), (18), (17), (17), (15), (14), (12), (10), (8), (8), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(331), (151), (100), (8), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(256), (68), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(18), (17), (17), (11), (10), (10), (8), (8), (8), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(264), (137), (95), (85), (79), (64), (52), (35), (32), (14), (13), (4), (3), (3), (2)
Pubmed 20 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN4343147) Images
Purification Immunogen affinity purified
Immunogen A genomic peptide made to c-t of the human p62/SQSTM1 protein (within residues 300-440). [Swiss-Prot Q13501]

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 antibody (ABIN4343147) Images back to top
Antigen
Alternative Name p62/SQSTM1 (SQSTM1 Antibody Abstract)
Background Gene Symbol: SQSTM1
Gene ID 8878
UniProt Q13501, Q64337, O08623
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 antibody (ABIN4343147) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:25-1:200, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenUse of this antibody in IHC-Frozen is reported in PMID 25014022 and in IHC-paraffin is reported in PMID 27497324. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 antibody (ABIN4343147) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-SQSTM1 antibody (ABIN4343147)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Eritja, Chen, Rodríguez-Barrueco, Santacana, Gatius, Vidal, Martí, Ponce, Bergadà, Yeramian, Encinas, Ribera, Reventós, Boyd, Villanueva, Matias-Guiu, Dolcet, Llobet-Navàs: "Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer." in: Autophagy, Vol. 13, Issue 3, pp. 608-624, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Su, Chu, Liao, Wang, Jan, Lin, Wu, Tseng, Yen, Huang: "Phellinus linteus Mycelium Alleviates Myocardial Ischemia-Reperfusion Injury through Autophagic Regulation." in: Frontiers in pharmacology, Vol. 8, pp. 175, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Kim, Jeong, Nah, Yoo, Lee, Kim, Moon, Hong, Jung: "Pimozide reduces toxic forms of tau in TauC3 mice via 5' adenosine monophosphate-activated protein kinase-mediated autophagy." in: Journal of neurochemistry, Vol. 142, Issue 5, pp. 734-746, 2017 Method employed by authors: Western Blotting (WB)

Lin: "In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy." in: Chemical research in toxicology, Vol. 28, Issue 2, pp. 202-15, 2016

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Sidjanin, Park, Ronchetti, Martins, Jackson: "TBC1D20 mediates autophagy as a key regulator of autophagosome maturation." in: Autophagy, Vol. 12, Issue 10, pp. 1759-1775, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Tan, Ho, Cho, Yu, Hung, Yu: "Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells." in: Cell death & disease, Vol. 7, Issue 8, pp. e2347, 2016 (Sample species: Human). Further details: Western Blotting

Song, Lee, Lim, Kim, Jang, Choi, Han: "Vibrio vulnificus VvhA induces autophagy-related cell death through the lipid raft-dependent c-Src/NOX signaling pathway." in: Scientific reports, Vol. 6, pp. 27080, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kim, Lee, Gabr, Choi, Kim, Ko, Han: "Aβ-Induced Drp1 phosphorylation through Akt activation promotes excessive mitochondrial fission leading to neuronal apoptosis." in: Biochimica et biophysica acta, Vol. 1863, Issue 11, pp. 2820-2834, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wang, Lin, Chang, Liu, Li, Kuo, Hsieh, Lee: "Hinokitiol induces autophagy in murine breast and colorectal cancer cells." in: Environmental toxicology, Vol. 31, Issue 1, pp. 77-84, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rožman, Yousefi, Oberson, Kaufmann, Benarafa, Simon: "The generation of neutrophils in the bone marrow is controlled by autophagy." in: Cell death and differentiation, Vol. 22, Issue 3, pp. 445-56, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sun, Chen, Tu, Wang, Chang, Li: "MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation." in: Bone, Vol. 73, pp. 145-53, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yao, Jia, Khan, Lin, Mitter, Boulton, Dunaief, Klionsky, Guan, Thompson, Zacks: "Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium." in: Autophagy, Vol. 11, Issue 6, pp. 939-53, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin, Jan, Kuo: "Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine." in: Molecular pharmaceutics, Vol. 12, Issue 8, pp. 2858-68, 2015

Dever, Rodriguez, Lapierre, Costin, El-Hage: "Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure." in: Frontiers in microbiology, Vol. 6, pp. 653, 2015 Further details: Immunocytochemistry,Immunofluorescence

Schwab, Ebert: "Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation." in: Stem cell reports, Vol. 5, Issue 6, pp. 1039-52, 2015 (Sample species: Human). Further details: Western Blotting

Nicks, Lee, Harris, Falk, Todd, Arredondo, Dunn, Notterpek: "Rapamycin improves peripheral nerve myelination while it fails to benefit neuromuscular performance in neuropathic mice." in: Neurobiology of disease, Vol. 70, pp. 224-36, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Fujiya, Konishi, Mohamed Kamel, Ueno, Inaba, Moriichi, Tanabe, Ikuta, Ohtake, Kohgo: "microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1." in: Oncogene, Vol. 33, Issue 40, pp. 4847-56, 2014 (Sample species: Human). Further details: Western Blotting

Rao, Tortola, Perlot, Wirnsberger, Novatchkova, Nitsch, Sykacek, Frank, Schramek, Komnenovic, Sigl, Aumayr, Schmauss, Fellner, Handschuh, Glösmann, Pasierbek, Schlederer, Resch, Ma, Yang, Popper et al.: "A dual role for autophagy in a murine model of lung cancer. ..." in: Nature communications, Vol. 5, pp. 3056, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lee, Lin, Liu, Chang, Hsieh, Wang: "Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway." in: Gene therapy, Vol. 21, Issue 3, pp. 309-16, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN4343147) back to top
Supplier Images
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 300-440) antibody (ABIN4343147) Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated wit...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (AA 300-440) antibody (ABIN4343147) Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - Immunocyto...
Simple Western (SimWes) image for anti-Sequestosome 1 (SQSTM1) (AA 300-440) antibody (ABIN4343147) Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Simple Western lane view shows a s...