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|Application / Reactivity||Human|
|Dot Blot (DB)||36 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||31 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||29 Antibodies|
|Immunofluorescence (IF)||62 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||144 Antibodies|
|Antigen||Sequestosome 1 (SQSTM1) Antibodies|
|Host||Guinea Pig Alternatives|
|Conjugate||This SQSTM1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
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Product Details anti-SQSTM1 AntibodyTarget Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN113450) Images
|Specificity||This antibody reacts with Human 62 kDa (p62) protein, present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. p62 protein (also described as ubiqutin-binding protein, sequestosome 1, SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy). The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse and Rat.|
|Immunogen||C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. Remarks: This peptide sequence is identical in Human, Monkey, Bovine, Mouse and Rat.|
Target Details SQSTM1Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN113450) Images back to top
|Alternative Name||SQSTM1 (SQSTM1 Antibody Abstract)|
|Background||Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62|
|Pathways||NF-kappaB Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN113450) Images back to top
Western blotting: 1/1,000 (ECL). Immunohistochemistry on Frozen Sections: 1/100Immunohistochemistry on Paraffin Embedded Sections (After formalin fixation): 1/100(microwave pretreatment improves staining). Incubation time: 1h at RT, extended with paraffin material.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN113450) Images back to top
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C Store after reconstition (in aliquots) at -20 °C.|
References for anti-SQSTM1 Antibody (ABIN113450)Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Stumptner, Fuchsbichler, Heid et al.: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, 2002 (PubMed).
Zatloukal, Stumptner, Fuchsbichler et al.: "p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases." in: The American journal of pathology, Vol. 160, Issue 1, pp. 255-63, 2002 (PubMed).
Stumptner, Heid, Fuchsbichler et al.: "Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1701-10, 1999 (PubMed).