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anti-Human SQSTM1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
AA 300-440
45
41
20
20
17
17
17
14
13
13
10
7
6
5
5
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
253
72
71
3
2
2
1
1
1
1
Host
Rabbit
197
49
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
14
13
12
8
8
8
6
6
6
4
4
3
3
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
180
98
77
63
50
48
37
36
20
11
9
3
3
2
Supplier
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Catalog No. ABIN4343147
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN113450 IHC (fro) IHC (p) WB Guinea Pig C-Term Log in to see Polyclonal 3
1 ABIN3043937 IHC (fro) IHC (p) WB Rabbit IgG AA 69-96, N-Term Log in to see Polyclonal
1 ABIN113451 IHC (fro) IHC (p) WB Guinea Pig N-Term Log in to see Polyclonal 3
1 ABIN334048 ICC IHC (fro) IHC (p) WB Guinea Pig IgG C-Term Log in to see Polyclonal
1 ABIN350827 IHC (fro) IHC (p) WB Rabbit AA 300-360 Log in to see Polyclonal 10
1 ABIN350826 IHC (fro) IHC (p) WB Sheep AA 300-360 Log in to see Polyclonal 10
1 ABIN350829 IHC (fro) IHC (p) WB Sheep IgG AA 300-360 Log in to see Polyclonal 10
1 ABIN350828 IHC (fro) IHC (p) WB Rabbit IgG AA 300-360 Log in to see Polyclonal 10
1 ABIN1860638 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 136-400 Log in to see Polyclonal
1 ABIN221787 IHC (fro) IHC (p) WB Guinea Pig IgG N-Term Log in to see Polyclonal
1 ABIN4962910 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962907 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962908 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962909 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962912 ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962914 ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962911 ICC IF IHC (fro) IHC (p) WB DyLight 650 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962906 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4343146 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal
1 ABIN4962913 IHC (fro) IHC (p) WB HRP Rabbit AA 300-440 Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-SQSTM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 7 Antibodies 7 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 35 Antibodies 36 Antibodies
Immunofluorescence (IF) 39 Antibodies 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope AA 300-440
(45), (41), (20), (20), (17), (17), (17), (14), (13), (13), (10), (7), (6), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(253), (72), (71), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(197), (49), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(14), (13), (12), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(180), (98), (77), (63), (50), (48), (37), (36), (20), (11), (9), (3), (3), (2)
Pubmed 20 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN4343147) Images
Purification Immunogen affinity purified
Immunogen A genomic peptide made to c-t of the human p62/SQSTM1 protein (within residues 300-440). [Swiss-Prot Q13501]

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN4343147) Images back to top
Antigen
Alternative Name p62/SQSTM1 (SQSTM1 Antibody Abstract)
Background Gene Symbol: SQSTM1
Gene ID 8878
UniProt Q13501, Q64337, O08623
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN4343147) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:25-1:200, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenUse of this antibody in IHC-Frozen is reported in PMID 25014022 and in IHC-paraffin is reported in PMID 27497324. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN4343147) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-SQSTM1 Antibody (ABIN4343147)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Lin: "In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy." in: Chemical research in toxicology, Vol. 28, Issue 2, pp. 202-15, 2016

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Sidjanin, Park, Ronchetti, Martins, Jackson: "TBC1D20 mediates autophagy as a key regulator of autophagosome maturation." in: Autophagy, Vol. 12, Issue 10, pp. 1759-1775, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Tan, Ho, Cho, Yu, Hung, Yu: "Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells." in: Cell death & disease, Vol. 7, Issue 8, pp. e2347, 2016 (Sample species: Human). Further details: Western Blotting

Wang, Lin, Chang, Liu, Li, Kuo, Hsieh, Lee: "Hinokitiol induces autophagy in murine breast and colorectal cancer cells." in: Environmental toxicology, Vol. 31, Issue 1, pp. 77-84, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rožman, Yousefi, Oberson, Kaufmann, Benarafa, Simon: "The generation of neutrophils in the bone marrow is controlled by autophagy." in: Cell death and differentiation, Vol. 22, Issue 3, pp. 445-56, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sun, Chen, Tu, Wang, Chang, Li: "MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation." in: Bone, Vol. 73, pp. 145-53, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yao, Jia, Khan, Lin, Mitter, Boulton, Dunaief, Klionsky, Guan, Thompson, Zacks: "Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium." in: Autophagy, Vol. 11, Issue 6, pp. 939-53, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin, Jan, Kuo: "Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine." in: Molecular pharmaceutics, Vol. 12, Issue 8, pp. 2858-68, 2015

Dever, Rodriguez, Lapierre, Costin, El-Hage: "Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure." in: Frontiers in microbiology, Vol. 6, pp. 653, 2015 Further details: Immunocytochemistry,Immunofluorescence

Schwab, Ebert: "Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation." in: Stem cell reports, Vol. 5, Issue 6, pp. 1039-52, 2015 (Sample species: Human). Further details: Western Blotting

Nicks, Lee, Harris, Falk, Todd, Arredondo, Dunn, Notterpek: "Rapamycin improves peripheral nerve myelination while it fails to benefit neuromuscular performance in neuropathic mice." in: Neurobiology of disease, Vol. 70, pp. 224-36, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Fujiya, Konishi, Mohamed Kamel, Ueno, Inaba, Moriichi, Tanabe, Ikuta, Ohtake, Kohgo: "microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1." in: Oncogene, Vol. 33, Issue 40, pp. 4847-56, 2014 (Sample species: Human). Further details: Western Blotting

Rao, Tortola, Perlot, Wirnsberger, Novatchkova, Nitsch, Sykacek, Frank, Schramek, Komnenovic, Sigl, Aumayr, Schmauss, Fellner, Handschuh, Glösmann, Pasierbek, Schlederer, Resch, Ma, Yang, Popper et al.: "A dual role for autophagy in a murine model of lung cancer. ..." in: Nature communications, Vol. 5, pp. 3056, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lee, Lin, Liu, Chang, Hsieh, Wang: "Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway." in: Gene therapy, Vol. 21, Issue 3, pp. 309-16, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin, Jan, Kuo: "Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine." in: Molecular pharmaceutics, Vol. 11, Issue 9, pp. 3002-8, 2014

Lu, Dong, Hao, Li, Zhu, Guo, Wang, Cheung, Wong, Wu, Markus, Yue: "Vacuolin-1 potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A." in: Autophagy, Vol. 10, Issue 11, pp. 1895-905, 2014

Pyo, Yoo, Ahn, Nah, Hong, Kam, Jung, Jung: "Overexpression of Atg5 in mice activates autophagy and extends lifespan." in: Nature communications, Vol. 4, pp. 2300, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mohammed, Fisher, Kraskauskas, Farkas, Brophy, Fowler, Natarajan: "Vitamin C: a novel regulator of neutrophil extracellular trap formation." in: Nutrients, Vol. 5, Issue 8, pp. 3131-51, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Greer, Yong, Fennell, Moustafa, Fowler, Galiano, Ng, Berkowitz, Cardelli, Meyers, Davis: "Knockdown of core binding factorβ alters sphingolipid metabolism." in: Journal of cellular physiology, Vol. 228, Issue 12, pp. 2350-64, 2013 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN4343147) back to top
Supplier Images
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 300-440) (ABIN4343147) Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated wit...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 300-440) (ABIN4343147) Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - Immunocyto...
Simple Western (SimWes) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 300-440) (ABIN4343147) Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Simple Western lane view shows a s...