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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||7 Antibodies||7 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies||10 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||8 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||24 Antibodies|
|Antigen||Sequestosome 1 (SQSTM1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SQSTM1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-SQSTM1 AntibodyTarget Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse and Rat|
|Immunogen||SQSTM1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of Human SQSTM1.|
Target Details SQSTM1Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
|Alternative Name||SQSTM1 (SQSTM1 Antibody Abstract)|
|Background||SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62|
|Pathways||NF-kappaB Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
ELISA. Western blot: SQSTM1 antibody can be used for the detection of SQSTM1 at 1-2 μg/mL. Positive Control: Human Spleen Tissue Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Upon receipt store the antibody (in aliquots) at -20 °C.|
References for anti-SQSTM1 Antibody (ABIN497992)Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Layfield, Ciani, Ralston et al.: "Structural and functional studies of mutations affecting the UBA domain of SQSTM1 (p62) which cause Paget's disease of bone." in: Biochemical Society transactions, Vol. 32, Issue Pt 5, pp. 728-30, 2004 (PubMed).
Seibenhener, Babu, Geetha et al.: "Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 8055-68, 2004 (PubMed).
Rousière, Michou, Cornélis et al.: "Paget's disease of bone." in: Best practice & research. Clinical rheumatology, Vol. 17, Issue 6, pp. 1019-41, 2004 (PubMed).
Hocking, Lucas, Daroszewska et al.: "Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease." in: Human molecular genetics, Vol. 11, Issue 22, pp. 2735-9, 2002 (PubMed).
Fillastre, Leroy, Josse et al.: "[Pharmacokinetics of trometamol-fosfomycin in patients with renal insufficiency]." in: Pathologie-biologie, Vol. 36, Issue 5 Pt 2, pp. 728-30, 1988 (PubMed).
ImagesProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) back to top