anti-Human SQSTM1 antibody for Immunoprecipitation

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
Internal Region
51
39
23
19
19
18
18
17
17
17
15
14
11
11
8
7
6
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
306
132
99
8
5
5
5
4
4
2
2
2
2
2
1
1
1
1
Host
Rabbit
251
48
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
17
16
16
10
10
10
7
7
7
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
245
121
84
81
65
64
35
35
20
13
13
4
4
2
2
Options
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Catalog No. ABIN2854836
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4343148 IHC IHC (p) IP SimWes WB Rabbit AA 325-375 Log in to see Polyclonal 1
1 ABIN4343150 ICC IF IHC IHC (p) IP WB Rabbit AA 425-440 Log in to see Polyclonal 3
1 ABIN4343149 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal
1 ABIN1874934 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1860638 ICC IHC IP WB Rabbit IgG AA 136-400 Log in to see Polyclonal
1 ABIN2887995 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2701519 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1920950 IF IP IHC (p) WB Rabbit IgG AA 425-440 Log in to see Polyclonal
1 ABIN1920949 IF IP IHC (p) WB Rabbit IgG AA 375-425 Log in to see Polyclonal
1 ABIN2988782 FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN5557948 ELISA IHC IP WB FITC Rabbit Log in to see Polyclonal
1 ABIN5557947 ELISA IHC IP WB Biotin Rabbit Log in to see Polyclonal
1 ABIN5557946 ELISA IHC IP WB Rabbit Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Immunoprecipitation

Similar anti-SQSTM1 Antibodies

Application / Reactivity Zebrafish (Danio rerio) Rat (Rattus)
Flow Cytometry (FACS) 1 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 53 Antibodies
Immunofluorescence (IF) 1 Antibodies 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 26 Antibodies
Immunoprecipitation (IP) 1 Antibodies 9 Antibodies
Western Blotting (WB) 1 Antibodies 85 Antibodies
ELISA 11 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Simple Western (SimWes) 3 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region
(51), (39), (23), (19), (19), (18), (18), (17), (17), (17), (15), (14), (11), (11), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(306), (132), (99), (8), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(251), (48), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(17), (16), (16), (10), (10), (10), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(245), (121), (84), (81), (65), (64), (35), (35), (20), (13), (13), (4), (4), (2), (2)
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (93 %)
Characteristics Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
SQSTM1 antibody [N3C1], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1. The exact sequence is proprietary.
Isotype IgG

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Cellular Localization: Cytoplasm , Late endosome , Nucleus
Molecular Weight 48 kDa
Gene ID 8878
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: A549 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 (untreated) , Huh7 ( 3uM Thapsigargin treatment for 12hr) , *HeLa

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot anal...
Immunohistochemistry (IHC) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on h...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot anal...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Whole cell ...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Flow Cytometry (FACS) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN2854836) FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometr...