anti-Human SQSTM1 antibody for Western Blotting

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
Internal Region, AA 350-400
52
39
22
19
19
18
18
17
17
16
15
14
12
11
8
7
6
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
325
150
99
8
5
5
5
4
4
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
252
66
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
18
17
17
11
10
10
8
8
8
6
6
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
260
137
95
84
80
65
52
35
33
14
13
4
4
3
2
Supplier
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Catalog No. ABIN449244
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9058776 ABIN449246 ICC IF SimWes WB Rabbit Internal Region, AA 400-450 Log in to see Polyclonal 1
2.9058776 ABIN388979 IF IHC (p) WB Rabbit Ig AA 317-346, C-Term Log in to see Polyclonal 22
2.9058776 ABIN682153 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal 4
2.9058776 ABIN4343147 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 29
2.9058776 ABIN1881829 WB Rabbit AA 383-409, C-Term, Ser403 Log in to see Polyclonal 5
2.9058776 ABIN4343162 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 AA 100-400 Log in to see 5H7E2 1
2.9058776 ABIN3044026 ICC IHC (p) WB Rabbit IgG AA 91-110, N-Term Log in to see Polyclonal
2.9058776 ABIN4343148 IHC IHC (p) IP SimWes WB Rabbit AA 325-375 Log in to see Polyclonal 1
2.9058776 ABIN4343150 ICC IF IHC IHC (p) IP WB Rabbit AA 425-440 Log in to see Polyclonal 3
2.9058776 ABIN2854836 ICC FACS IF IP IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9058776 ABIN615711 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
2.9058776 ABIN1537711 WB Rabbit Ig AA 100-127, Center Log in to see Polyclonal
2.9058776 ABIN2737581 IHC WB Rabbit IgG Log in to see Polyclonal
2.9058776 ABIN4343149 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal
2.9058776 ABIN1874934 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
2.9058776 ABIN3021065 IHC WB Rabbit IgG Log in to see Polyclonal
2.9058776 ABIN4343163 WB Rabbit pSer272, pThr269 Log in to see Polyclonal
2.9058776 ABIN1860638 ICC IHC IP WB Rabbit IgG AA 136-400 Log in to see Polyclonal
2.9058776 ABIN3032535 ELISA WB Rabbit Ig Fraction AA 383-409 Log in to see Polyclonal
2.9058776 ABIN5078601 ICC IF WB Rabbit IgG Log in to see Polyclonal

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region, AA 350-400
(52), (39), (22), (19), (19), (18), (18), (17), (17), (16), (15), (14), (12), (11), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(325), (150), (99), (8), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(252), (66), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(18), (17), (17), (11), (10), (10), (8), (8), (8), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(260), (137), (95), (84), (80), (65), (52), (35), (33), (14), (13), (4), (4), (3), (2)
Pubmed 1 reference available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN449244) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the human p62/SQSTM1 protein (within residues 350-400). [Swiss-Prot Q13501]

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 antibody (ABIN449244) Images back to top
Antigen
Alternative Name p62/SQSTM1 (SQSTM1 Antibody Abstract)
Background Gene Symbol: SQSTM1
Molecular Weight Theoretical MW: 47 kDa
Gene ID 8878
UniProt Q13501, Q64337, O08623
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 antibody (ABIN449244) Images back to top
Application Notes Western Blot 0.5 μg/mL, Immunocytochemistry/Immunofluorescence 1:50
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for SQSTM1 Antibody Procedure Guide for NBP1-42821 - SQSTM1 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-SQSTM1 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 antibody (ABIN449244) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-SQSTM1 antibody (ABIN449244)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Eason, Bell, Marshall, Rodgers, Pineda, Steiger, Al-Riyami, Harnett, Harnett: "The helminth product, ES-62 modulates dendritic cell responses by inducing the selective autophagolysosomal degradation of TLR-transducers, as exemplified by PKCδ." in: Scientific reports, Vol. 6, pp. 37276, 2016 Method employed by authors: Chromatin Immunoprecipitation (ChIP)

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN449244) back to top
Supplier Images
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 350-400), (Internal Region) antibody (ABIN449244) anti-Sequestosome 1 (SQSTM1) (AA 350-400), (Internal Region) antibody
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 350-400), (Internal Region) antibody (ABIN449244) Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or ...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (AA 350-400), (Internal Region) antibody (ABIN449244) Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - SQSTM1 sta...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (AA 350-400), (Internal Region) antibody (ABIN449244) Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal i...