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anti-Human SQSTM1 antibody for Western Blotting

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
AA 317-346, C-Term
53
40
23
20
20
18
18
17
17
17
15
14
12
11
8
7
6
5
5
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
304
132
99
8
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
248
49
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
16
16
15
10
10
10
7
7
7
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
241
124
82
81
65
64
37
37
20
13
11
4
4
3
2
Options
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Catalog No. ABIN388979
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN497992 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 5
2.9590726 ABIN4620406 EIA IF IHC (p) WB Rabbit Ig AA 324-353, C-Term Log in to see Polyclonal 12
2.9590726 ABIN449246 ICC IF SimWes WB Rabbit Internal Region, AA 400-450 Log in to see Polyclonal 1
2.9590726 ABIN449244 ICC IF WB Rabbit Internal Region, AA 350-400 Log in to see Polyclonal 1
2.9590726 ABIN1881829 WB Rabbit AA 383-409, C-Term, Ser403 Log in to see Polyclonal 5
2.9590726 ABIN682153 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal 4
2.9590726 ABIN4343148 IHC IHC (p) IP SimWes WB Rabbit AA 325-375 Log in to see Polyclonal 1
2.9590726 ABIN4343150 ICC IF IHC IHC (p) IP WB Rabbit AA 425-440 Log in to see Polyclonal 3
2.9590726 ABIN4343147 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 24
2.9590726 ABIN2785619 IHC WB Rabbit Middle Region Log in to see Polyclonal
2.9590726 ABIN3043937 IHC (fro) IHC (p) WB Rabbit IgG AA 69-96, N-Term Log in to see Polyclonal
2.9590726 ABIN2854836 ICC FACS IF IP IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590726 ABIN3044026 ICC IHC (p) WB Rabbit IgG AA 91-110, N-Term Log in to see Polyclonal
2.9590726 ABIN615711 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
2.9590726 ABIN1537711 WB Rabbit Ig AA 100-127, Center Log in to see Polyclonal
2.9590726 ABIN4343162 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 AA 100-400 Log in to see 5H7E2
2.9590726 ABIN1874934 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN2737581 IHC WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN4343149 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal
2.9590726 ABIN3021066 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Western Blotting

Similar anti-SQSTM1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 37 Antibodies
ELISA 81 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 82 Antibodies
Immunofluorescence (IF) 125 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 242 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope AA 317-346, C-Term
(53), (40), (23), (20), (20), (18), (18), (17), (17), (17), (15), (14), (12), (11), (8), (7), (6), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(304), (132), (99), (8), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(248), (49), (4), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This SQSTM1 antibody is un-conjugated
(16), (16), (15), (10), (10), (10), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(241), (124), (82), (81), (65), (64), (37), (37), (20), (13), (11), (4), (4), (3), (2)
Pubmed 20 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN388979) Images
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 AA from the C-terminal region of human SQSTM1 (p62).
Clone RB4614
Isotype Ig

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN388979) Images back to top
Antigen
Alternative Name SQSTM1 (p62) (SQSTM1 Antibody Abstract)
Background SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

Synonyms: Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
Pathways: Osteoclast differentiation
Molecular Weight 47687 Da
Gene ID 8878
UniProt Q13501
Research Area Ubiquitin-related antibodies, Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Cycle, Autophagy, Cell Structure
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN388979) Images back to top
Application Notes Suggested dilutions:
IF = 1:50-100, WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN388979) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-SQSTM1 Antibody (ABIN388979)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Tan, Joshua, Saggar, Yu, Wang, Kanga, Zhang, Chen, Wouters, Tannock: "Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy." in: British journal of cancer, Vol. 112, Issue 5, pp. 832-40, 2015

Wu, Zhang, Lu, Xia, Zhou, Petrof, Claud, Chen, Chang, Carmeliet, Sun: "Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis." in: Gut, Vol. 64, Issue 7, pp. 1082-94, 2015

Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, 2015

Zhang, Li, Wang, Chen, Dong, Nie: "Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis." in: Tumour biology, 2015

Gurpinar, Grizzle, Shacka, Mader, Li, Piazza, Russo, Keeton, Piazza: "A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy." in: Molecular cancer therapeutics, Vol. 12, Issue 5, pp. 663-74, 2013

Urtishak, Edwards, Wang, Hudome, Robinson, Barrett, Cao, Cory, Moore, Bantly, Yu, Chen, Atlas, Willman, Kundu, Carroll, Heerema, Devidas, Hilden, Dreyer, Hunger, Reaman, Felix: "Potent obatoclax cytotoxicity and activation of triple death mode killing across infant acute lymphoblastic leukemia." in: Blood, Vol. 121, Issue 14, pp. 2689-703, 2013

Han, Pan, Xu, Xiao, An, Tie, Pan, Li: "Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage." in: Autophagy, Vol. 8, Issue 5, pp. 812-25, 2012

Soontornniyomkij, Risbrough, Young, Soontornniyomkij, Jeste, Achim: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, 2012

Lee, Shin, Yuk, Shi, Choi, Lee, Huang, Kim, Kim, Lee, Jo: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, 2011

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, 2011

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, 2011

Zhang, Lowry, Guarente, Haimovich: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, 2010

Maynard, Dvorak, Khailova, Dobrenen, Arganbright, Halpern, Kurundkar, Maheshwari, Dvorak: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, 2010

Lin, Whang, Donner, Du, Lorch, He, Jiang, Price, Moore, Ruan: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, 2010

Chu, Wang, Wu, Lei: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, 2010

Wang, Gorvel, Chu, Wu, Lei: "Helicobacter pylori impairs murine dendritic cell responses to infection." in: PLoS ONE, Vol. 5, Issue 5, pp. e10844, 2010

Kobayashi, Volden, Timm, Mao, Xu, Liang: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, 2009

Tal, Sasai, Lee, Yordy, Shadel, Iwasaki: "Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 8, pp. 2770-5, 2009

Ju, Miller, Hanson, Weihl: "Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease." in: The Journal of biological chemistry, Vol. 283, Issue 44, pp. 30289-99, 2008

Background publications

Seibenhener, Babu, Geetha, Wong, Krishna, Wooten: "Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 8055-68, 2004

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN388979) back to top
Supplier Images
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 317-346) (ABIN388979) Western blot analysis of hSQSTM1-C331 (ABIN388979) in A2058 cell line lysates (35 µg/...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 317-346) (ABIN388979) Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62...
Immunohistochemistry (IHC) image for anti-SQSTM1 antibody (Sequestosome 1) (AA 317-346) (ABIN388979) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...