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|Antigen||Sequestosome 1 (SQSTM1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SQSTM1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-SQSTM1 AntibodyTarget Details SQSTM1 Application Details Handling Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse and Rat|
|Immunogen||SQSTM1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of Human SQSTM1.|
Target Details SQSTM1Product Details anti-SQSTM1 Antibody Application Details Handling Images back to top
|Alternative Name||SQSTM1 (SQSTM1 Antibody Abstract)|
|Background||SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62|
|Pathways||NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy|
Application DetailsProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling Images back to top
ELISA. Western blot: SQSTM1 antibody can be used for the detection of SQSTM1 at 1-2 μg/mL. Positive Control: Human Spleen Tissue Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Upon receipt store the antibody (in aliquots) at -20 °C.|