anti-Rat (Rattus) SQSTM1 antibody for Immunofluorescence

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Epitope
Internal Region, AA 400-450
51
39
23
19
19
18
18
17
17
16
15
14
11
11
8
7
6
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
306
133
100
8
5
5
5
4
4
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
250
49
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
17
16
16
10
10
10
7
7
7
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
245
122
83
81
65
65
37
35
20
14
13
4
3
2
2
Supplier
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Catalog No. ABIN449246
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.16076 ABIN449244 ICC IF WB Rabbit Internal Region, AA 350-400 Log in to see Polyclonal 1
12.16076 ABIN4343147 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 27
12.16076 ABIN2854836 ICC FACS IF IP IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1874934 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2572550 FACS IF IHC (p) WB Mouse IgG2b Log in to see 1E9
1 ABIN4343152 ICC IF WB DyLight 650 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4343153 ICC IF WB DyLight 488 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4343159 ICC IF WB DyLight 405 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4236622 ICC IF FITC Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4236623 ICC IF FITC Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343158 ICC IF WB DyLight 550 Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343168 ICC IF WB Alexa Fluor 405 Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343151 ICC IF WB Biotin Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4343154 ICC IF WB DyLight 550 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4343157 ICC IF WB DyLight 488 Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343155 ICC IF WB Biotin Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343156 ICC IF WB DyLight 650 Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343160 ICC IF WB DyLight 405 Rabbit AA 400-450, Internal Region Log in to see Polyclonal
1 ABIN4343164 ICC IF WB Alexa Fluor 405 Rabbit AA 350-400, Internal Region Log in to see Polyclonal
1 ABIN4343165 ICC IF WB Alexa Fluor 488 Rabbit AA 350-400, Internal Region Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Immunofluorescence

Similar anti-SQSTM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 11 Antibodies 11 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 7 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 53 Antibodies 54 Antibodies
Immunofluorescence (IF) 60 Antibodies 60 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region, AA 400-450
(51), (39), (23), (19), (19), (18), (18), (17), (17), (16), (15), (14), (11), (11), (8), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(306), (133), (100), (8), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(250), (49), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(17), (16), (16), (10), (10), (10), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
(245), (122), (83), (81), (65), (65), (37), (35), (20), (14), (13), (4), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling ProductDetails: References for anti-SQSTM1 antibody (ABIN449246) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the human p62/SQSTM1 protein (within residues 400-450). [Swiss-Prot Q13501]

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling ProductDetails: References for anti-SQSTM1 antibody (ABIN449246) Images back to top
Antigen
Alternative Name p62/SQSTM1 (SQSTM1 Antibody Abstract)
Background Gene Symbol: SQSTM1
Molecular Weight Theoretical MW: 47 kDa
Gene ID 8878
UniProt Q13501
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling ProductDetails: References for anti-SQSTM1 antibody (ABIN449246) Images back to top
Application Notes Western Blot 1:2000, Simple Western 1:25, Immunocytochemistry/Immunofluorescence 1:50In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for SQSTM1 Antibody Procedure Guide for NBP1-42822 - SQSTM1 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-SQSTM1 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details ProductDetails: References for anti-SQSTM1 antibody (ABIN449246) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-SQSTM1 antibody (ABIN449246)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Zhang, Gui, Huang, Deng, Fang, Ke, He, Li, Fang: "Neuroprotective Effects of β-Asarone Against 6-Hydroxy Dopamine-Induced Parkinsonism via JNK/Bcl-2/Beclin-1 Pathway." in: Molecular neurobiology, Vol. 53, Issue 1, pp. 83-94, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling ProductDetails: References for anti-SQSTM1 antibody (ABIN449246) back to top
Supplier Images
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN449246) anti-Sequestosome 1 (SQSTM1) (AA 400-450), (Internal Region) antibody
Simple Western (SimWes) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN449246) Simple Western: p62/SQSTM1 Antibody [NBP1-42822] - Simple Western lane view shows a s...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN449246) Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - HeLa cells were treated with (+) or ...
Immunofluorescence (IF) image for anti-SQSTM1 antibody (Sequestosome 1) (Internal Region) (ABIN449246) Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42822] - staining i...