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anti-Rat (Rattus) SQSTM1 antibody for Western Blotting

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • A170
  • MGC79491
  • OSF-6
  • Osi
  • OSIL
  • p60
  • p62
  • p62B
  • PDB3
  • sb:cb621
  • sqstm1
  • SQSTM1
  • STAP
  • zgc:85784
  • ZIP
  • ZIP3
Epitope
C-Term
44
41
19
19
18
17
17
14
13
13
7
6
5
5
5
5
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
238
61
60
3
2
2
1
1
1
Host
Rabbit
184
47
4
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
13
12
12
8
8
8
5
5
5
3
3
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
89
77
56
48
42
36
34
12
11
8
4
2
2
Supplier
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Catalog No. ABIN497992
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN682153 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal 1
0.13341247 ABIN2785619 IHC WB Rabbit Middle Region Log in to see Polyclonal
0.13341247 ABIN3043937 IHC (fro) IHC (p) WB Rabbit IgG AA 69-96, N-Term Log in to see Polyclonal
0.13341247 ABIN615711 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2854836 ICC FACS IF IP IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN3044026 ICC IHC (p) WB Rabbit IgG AA 91-110, N-Term Log in to see Polyclonal
0.13341247 ABIN449247 ICC IF SimWes WB Rabbit Internal Region, AA 400-450 Log in to see Polyclonal 1
0.13341247 ABIN449245 ICC IF WB Rabbit Internal Region, AA 350-400 Log in to see Polyclonal
0.13341247 ABIN1874934 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3021066 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1030699 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2505882 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN682155 IHC (p) WB Biotin Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0011728023 ABIN682162 IHC (p) WB HRP Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0011728023 ABIN1739629 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011728023 ABIN634293 WB Rabbit Middle Region Log in to see Polyclonal
0.0011728023 ABIN2737581 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1092121 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1092122 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1886964 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-SQSTM1 antibody for Western Blotting

Similar anti-SQSTM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 7 Antibodies 7 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 27 Antibodies 28 Antibodies
Immunofluorescence (IF) 29 Antibodies 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 13 Antibodies 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope C-Term
(44), (41), (19), (19), (18), (17), (17), (14), (13), (13), (7), (6), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(238), (61), (60), (3), (2), (2), (1), (1), (1)
Host Rabbit
(184), (47), (4), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(13), (12), (12), (8), (8), (8), (5), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(172), (89), (77), (56), (48), (42), (36), (34), (12), (11), (8), (4), (2), (2)
Pubmed 5 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) Images
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat
Purification Immunoaffinity Chromatography
Immunogen SQSTM1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of Human SQSTM1.
Isotype IgG

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62
Gene ID 8878
NCBI Accession NP_001135770
UniProt Q13501
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
Application Notes ELISA. Western blot: SQSTM1 antibody can be used for the detection of SQSTM1 at 1-2 μg/mL. Positive Control: Human Spleen Tissue Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 Antibody (ABIN497992) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt store the antibody (in aliquots) at -20 °C.

References for anti-SQSTM1 Antibody (ABIN497992)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Background publications

Layfield, Ciani, Ralston, Hocking, Sheppard, Searle, Cavey: "Structural and functional studies of mutations affecting the UBA domain of SQSTM1 (p62) which cause Paget's disease of bone." in: Biochemical Society transactions, Vol. 32, Issue Pt 5, pp. 728-30, 2004

Seibenhener, Babu, Geetha, Wong, Krishna, Wooten: "Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 8055-68, 2004

Rousière, Michou, Cornélis, Orcel: "Paget's disease of bone." in: Best practice & research. Clinical rheumatology, Vol. 17, Issue 6, pp. 1019-41, 2004

Hocking, Lucas, Daroszewska, Mangion, Olavesen, Cundy, Nicholson, Ward, Bennett, Wuyts, Van Hul, Ralston: "Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease." in: Human molecular genetics, Vol. 11, Issue 22, pp. 2735-9, 2002

Fillastre, Leroy, Josse, Moulin: "[Pharmacokinetics of trometamol-fosfomycin in patients with renal insufficiency]." in: Pathologie-biologie, Vol. 36, Issue 5 Pt 2, pp. 728-30, 1988

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 Antibody (ABIN497992) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SQSTM1 antibody (Sequestosome 1) (C-Term) (ABIN497992) AP20066PU-N SQSTM1 antibody staining of Rat Spleen tissue by Immunohistochemistry at ...
Western Blotting (WB) image for anti-SQSTM1 antibody (Sequestosome 1) (C-Term) (ABIN497992) AP20066PU-N SQSTM1 antibody staining of Human Spleen Tissue Lysate by Western Blottin...