Human SQSTM1 ELISA Kit

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Antigen
Sequestosome 1 (SQSTM1) ELISA Kits
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
5
2
1
Method Type
Sandwich ELISA
Detection Range
0.312-20 ng/mL
Minimum Detection Limit
0.312 ng/mL
Application
ELISA
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Catalog No. ABIN1572524
$ 908.41
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN418889 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
1 ABIN5658717 Sandwich ELISA Biological Fluids, Cell Lysate, Tissue Homogenate 0.312 ng/mL - 20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2538896 Sandwich ELISA Tissue Homogenate, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN4972738 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN1156930 Log in to see

General

Antigen Sequestosome 1 (SQSTM1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(5), (2), (1)
Method Type Sandwich ELISA
Detection Range 0.312-20 ng/mL
Minimum Detection Limit 0.312 ng/mL
Application ELISA
Supplier Log in to see

Product Details SQSTM1 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SQSTM1 in human tissue homogenates, cell lysates and other biological fluids.
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.125 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details SQSTM1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 ELISA Kit Abstract)
Background Synonyms: EBI3-associated protein of 60 kDa,EBIAP,ORCA,OSIL,p60,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Sequestosome-1,Ubiquitin-binding protein
Gene ID 8878
UniProt Q13501
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details SQSTM1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details SQSTM1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details SQSTM1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Sequestosome 1 (SQSTM1) ELISA Kit (ABIN1572524) Sequestosome 1 (SQSTM1) ELISA Kit