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anti-Cow (Bovine) TGFA antibody for Immunohistochemistry (Frozen Sections)

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TFGA
  • TGFA
  • TGFAA
  • TGF alpha
  • wa-1
  • wa1
Reactivity
Cow (Bovine), Human, Rat (Rattus)
172
64
37
13
10
9
4
2
1
1
Host
Sheep
132
43
20
4
1
Clonality
Polyclonal
Conjugate
This TGFA antibody is un-conjugated
20
8
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
110
98
63
32
24
24
19
16
11
10
7
6
4
4
3
2
2
1
Supplier
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Catalog No. ABIN336238
$ 632.50
Plus shipping costs $45.00

Top referenced anti-TGFA antibody for Immunohistochemistry (Frozen Sections)

Similar anti-TGFA Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 25 Antibodies 81 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 7 Antibodies
Flow Cytometry (FACS) 6 Antibodies 22 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 31 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Functional Studies (Func) 4 Antibodies

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Reactivity Cow (Bovine), Human, Rat (Rattus)
(172), (64), (37), (13), (10), (9), (4), (2), (1), (1)
Host Sheep
(132), (43), (20), (4), (1)
Clonality
Polyclonal
Conjugate This TGFA antibody is un-conjugated
(20), (8), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(110), (98), (63), (32), (24), (24), (19), (16), (11), (10), (7), (6), (4), (4), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling References for anti-TGFA Antibody (ABIN336238) Images
Specificity This antibody Reacts with TGFα and Does not react with EGF or TGFβ. Human uterous, ovary or foreskin.
Purification Serum
Immunogen Recombinant Mature human TGFalpha.

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling References for anti-TGFA Antibody (ABIN336238) Images back to top
Antigen
Alternative Name TGF-alpha (TGFA) (TGFA Antibody Abstract)
Background Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33 % homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha
Gene ID 7039
NCBI Accession NP_001093161
UniProt P01135
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling References for anti-TGFA Antibody (ABIN336238) Images back to top
Application Notes Immunoprecipitation: This antibody has been used at dilutions from 1/250-1/1000 toimmunoprecipitate mature and higher molecular weight forms of human TGFα from thetissue culture fluid of metabolically labelled cells over-expressing the full length TGFαprecursor molecule as well as from rat and bovine tissue preparations. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: Reacts withparaffin-fixed human uterus, ovary and foreskin at 1/25-1/100. In ELISA with 1 μg/mL synthetic TGFα in the coating buffer the end point dilution was1/16,000. Homologous RIA titres range from 1/30k-1/100k (human and bovine) and1/2k-1/10k (rat) at a sensitivity of 0.2 ng minimum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details References for anti-TGFA Antibody (ABIN336238) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-TGFA Antibody (ABIN336238)

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling Images back to top
Background publications

Ju, Velu, Vass, Papageorge, Lowy: "Tumorigenic transformation of NIH 3T3 cells by the autocrine synthesis of transforming growth factor alpha." in: The New biologist, Vol. 3, Issue 4, pp. 380-8, 1991