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anti-Human TGFA antibody for Blocking Antibody

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TFGA
  • TGFA
  • TGFAA
  • TGF alpha
  • wa-1
  • wa1
Reactivity
Human
330
124
121
86
59
32
4
2
1
1
Host
Rabbit
201
131
21
4
1
Clonality
Polyclonal
Conjugate
This TGFA antibody is un-conjugated
27
13
10
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
198
108
102
95
77
67
63
58
55
17
11
8
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN1003355
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086317887 ABIN289578 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Similar anti-TGFA Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 91 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 93 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 53 Antibodies
Immunofluorescence (IF) 76 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 94 Antibodies
Immunoprecipitation (IP) 64 Antibodies
Neutralization (Neut) 17 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Reactivity Human
(330), (124), (121), (86), (59), (32), (4), (2), (1), (1)
Host Rabbit
(201), (131), (21), (4), (1)
Clonality
Polyclonal
Conjugate This TGFA antibody is un-conjugated
(27), (13), (10), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(198), (108), (102), (95), (77), (67), (63), (58), (55), (17), (11), (8), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hTGFalpha (human Transforming Growth Factor-alpha)
Isotype IgG

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name Transforming Growth Factor alpha (TGFA Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGFalpha (human Transforming Growth Factor-alpha). Anti-Human TGFalpha specific antibody was purified by affinity chromatography employing immobilized hTGFalpha matrix.
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hTGF-alpha (0.5 ng/µl), a concentration of 2.5 - 3.7 µg/µl of this antibody is required. To detect hTGF-alpha by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-alpha. To detect hTGF-alpha by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human TGF-alpha (PK-AB815-63535B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-alpha. To detect hTGFalpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFalpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect hTGF-alpha by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human TGF-alpha (PK-AB815-63535B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-alpha. To detect hTGFalpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFalpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of hTGF-alpha (0.5 ng/µl), a concentration of 2.5 - 3.7 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months