anti-Rabbit TGFA antibody for Immunohistochemistry

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TGFA
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TGFAA
  • wa-1
  • wa1
  • TFGA
  • TGF alpha
  • transforming growth factor, alpha
  • transforming growth factor alpha
  • transforming growth factor-alpha
  • tgfa
  • TGFA
  • LOC100009482
  • LOC100121300
  • Tgfa
  • TGF-A
  • LOC100009150
  • LOC100358936
Epitope
AA 34-43
100
77
16
6
5
5
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rabbit, Zebrafish
341
120
116
93
71
37
6
3
2
2
Host
Mouse
204
138
17
4
1
Clonality
Monoclonal
Conjugate
This TGFA antibody is un-conjugated
26
13
10
9
8
8
7
7
7
7
7
7
7
7
7
7
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
179
174
164
158
145
116
114
64
62
18
14
13
8
4
4
2
2
2
1
1
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Catalog No. ABIN3026163
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.894592 ABIN3026446 FACS IF IHC Mouse IgM, kappa AA 34-43 Log in to see SPM542
8.894592 ABIN1585841 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN3026164 FACS IF IHC Mouse IgM, kappa AA 34-43 Log in to see 1E8-G6
1 ABIN3026447 FACS IF IHC Mouse IgM, kappa AA 34-43 Log in to see SPM542

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope AA 34-43
(100), (77), (16), (6), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rabbit, Zebrafish
(341), (120), (116), (93), (71), (37), (6), (3), (2), (2)
Host Mouse
(204), (138), (17), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFA antibody is un-conjugated
(26), (13), (10), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(179), (174), (164), (158), (145), (116), (114), (64), (62), (18), (14), (13), (8), (4), (4), (2), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Purification PEG precipitation
Immunogen A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.
Clone 1E8-G6
Isotype IgM, kappa

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name TGF alpha (TGFA Antibody Abstract)
Background TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Gene ID 7039
Research Area Cancer, Angiogenesis, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Transforming Growth Factor, alpha (TGFA) (AA 34-43) antibody (ABIN3026163) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha a...