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Human TGFA ELISA Kit

Recommended TGFA Product (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) ELISA Kits
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TFGA
  • TGFA
  • TGFAA
  • TGF alpha
  • wa-1
  • wa1
Epitope
AA 40-89
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
28
15
15
6
6
4
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921075
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.31757757 ABIN924864 Sandwich ELISA Cell Lysate, Serum, Plasma 8-500 pg/mL 8 pg/mL Log in to see
0.31757757 ABIN414921 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.31757757 ABIN1888492 Sandwich ELISA Serum, Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Milk 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.31757757 ABIN2708380 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.31757757 ABIN2115067 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Milk Log in to see
0.0012704609 ABIN365401 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012704609 ABIN509603 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012704609 ABIN625093 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 3-1000 pg/mL 3 pg/mL Log in to see 1
0.0012704609 ABIN1572654 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012704609 ABIN1381544 Cell ELISA Log in to see
0.0012704609 ABIN509600 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012704609 ABIN1112790 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012704609 ABIN2115066 Cell Lysate, Serum, Plasma 8-500 pg/mL 8 pg/mL Log in to see
0.0012704609 ABIN2042674 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012704609 ABIN2685614 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012704609 ABIN2685613 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum, Milk 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012704609 ABIN2115068 Cell ELISA Log in to see
0.0012704609 ABIN1847053 Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012704609 ABIN2539848 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012704609 ABIN2685615 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see

Top referenced TGFA ELISA Kit for ELISA

  • Human TGFA ELISA Kit for Sandwich ELISA - ABIN921075 (8 Publications): Sasaki, Kuniyasu, Luo, Kato, Shinya, Fujii, Ohmori, Yamashita: Significance of epithelial growth factor in the epithelial-mesenchymal transition of human gallbladder cancer cells. in Cancer science 2012 (PubMed)

  • Human TGFA ELISA Kit for Sandwich ELISA - ABIN625093 (1 Publications): Acocella, Bertrand, Beytout, Durrande, Garcia Rodriguez, Kosmidis, Micoud, Rey, Rodriguez Zapata, Roux: Comparison of three different regimens in the treatment of acute brucellosis: a multicenter multinational study. in The Journal of antimicrobial chemotherapy 1989 (PubMed)

Similar TGFA ELISA Kits

Application / Reactivity Human
ELISA 26 ELISA Kits

General

Antigen Transforming Growth Factor, alpha (TGFA) ELISA Kits
Epitope AA 40-89
Reactivity Human
Kits with alternative reactivity to:
(28), (15), (15), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 8 references available
Supplier Log in to see

Product Details TGFA ELISA Kit

Target details Application Details Handling References for TGFA Kit (ABIN921075) Images
Purpose For quantitative detection of human TGF alpha in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk
Detection Method Colorimetric
Specificity Natural and recombinant human TGF alpha
Cross-Reactivity (Details) There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1 % .
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- human TGF alpha antibod - 1
  • Lyophilized recombinant human TGF alpha standar - 10ng/tubex2
  • Biotinylated anti- human TGF alpha antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: V40-A89

Target details

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Antigen
Alternative Name TGFA (TGFA ELISA Kit Abstract)
Background Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
Gene ID 7039
UniProt P01135
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF alpha amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 118, Standard deviation: 5.43, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 225, Standard deviation: 12.83, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 516, Standard deviation: 16.5, CV(%): 3.2,
  • Sample 1: n=24, Mean(pg/ml): 126, Standard deviation: 8.95, CV(%): 7.1
  • Sample 2: n=24, Mean(pg/ml): 238, Standard deviation: 14.28, CV(%): 6
  • Sample 3: n=24, Mean(pg/ml): 509, Standard deviation: 27, CV(%): 5.3
Restrictions For Research Use only

Handling

Product Details TGFA ELISA Kit Target details Application Details References for TGFA Kit (ABIN921075) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TGFA Kit (ABIN921075)

Product Details TGFA ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, 2015

Chen, Qi, Feng, Wang, Bao, Wang, Xiang, Xie: "Neuroprotective effect of allicin against traumatic brain injury via Akt/endothelial nitric oxide synthase pathway-mediated anti-inflammatory and anti-oxidative activities." in: Neurochemistry international, Vol. 68, pp. 28-37, 2014

Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, 2013

Huang, Zhang, Wang, Yu, Fang, Lu, Zhang, Wei: "Transforming growth factor β1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1." in: Journal of neuroinflammation, Vol. 9, pp. 145, 2012

Mao, Gao, Li, Li: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, 2011

Ye, Wu, Guo, Wang, Yuan, Shang, Yao: "Lipoxin A4 analogue protects brain and reduces inflammation in a rat model of focal cerebral ischemia reperfusion." in: Brain research, Vol. 1323, pp. 174-83, 2010

Background publications

Sasaki, Kuniyasu, Luo, Kato, Shinya, Fujii, Ohmori, Yamashita: "Significance of epithelial growth factor in the epithelial-mesenchymal transition of human gallbladder cancer cells." in: Cancer science, Vol. 103, Issue 6, pp. 1165-71, 2012

Fallon, Reid, Kinyamu, Opole, Opole, Baratta, Korc, Endo, Duong, Nguyen, Karkehabadhi, Twardzik, Patel, Loughlin: "In vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 26, pp. 14686-91, 2001

Images

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Supplier Images
ELISA image for TGFA ELISA Kit (Transforming Growth Factor, alpha) (ABIN921075) Human TGF alpha PicoKine ELISA Kit standard curve