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|Application / Reactivity||Human|
|ELISA||26 ELISA Kits|
|Antigen||Transforming Growth Factor, alpha (TGFA) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||15.6-1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|8 references available|
|Supplier||Log in to see|
Product Details TGFA ELISA KitTarget details Application Details Handling References for TGFA Kit (ABIN921075) Images
|Purpose||For quantitative detection of human TGF alpha in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk|
|Specificity||Natural and recombinant human TGF alpha|
|Cross-Reactivity (Details)||There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1 % .|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: E.coli
Immunogen sequence: V40-A89
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|Background||Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.|
|Pathways||NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||human TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF alpha amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
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|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for TGFA Kit (ABIN921075)Product Details TGFA ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, 2015 (PubMed).
Chen, Qi, Feng et al.: "Neuroprotective effect of allicin against traumatic brain injury via Akt/endothelial nitric oxide synthase pathway-mediated anti-inflammatory and anti-oxidative activities." in: Neurochemistry international, Vol. 68, pp. 28-37, 2014 (PubMed).
Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, 2013 (PubMed).
Huang, Zhang, Wang et al.: "Transforming growth factor β1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1." in: Journal of neuroinflammation, Vol. 9, pp. 145, 2012 (PubMed).
Mao, Gao, Li et al.: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, 2011 (PubMed).
Ye, Wu, Guo et al.: "Lipoxin A4 analogue protects brain and reduces inflammation in a rat model of focal cerebral ischemia reperfusion." in: Brain research, Vol. 1323, pp. 174-83, 2010 (PubMed).
Sasaki, Kuniyasu, Luo et al.: "Significance of epithelial growth factor in the epithelial-mesenchymal transition of human gallbladder cancer cells." in: Cancer science, Vol. 103, Issue 6, pp. 1165-71, 2012 (PubMed).
Fallon, Reid, Kinyamu et al.: "In vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 26, pp. 14686-91, 2001 (PubMed).
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