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Rhesus Monkey TGFA ELISA Kit

Recommended TGFA Product (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) ELISA Kits
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TFGA
  • TGFA
  • TGFAA
  • TGF alpha
  • wa-1
  • wa1
Reactivity
Rhesus Monkey
Alternatives
Kits with alternative reactivity to:
29
15
15
6
6
4
3
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.25-2000 pg/mL
Minimum Detection Limit
31.25 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1572658
$ 919.15
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.001212079 ABIN2748660 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see

Similar TGFA ELISA Kits

Application / Reactivity Rhesus Monkey
ELISA 2 ELISA Kits

General

Antigen Transforming Growth Factor, alpha (TGFA) ELISA Kits
Reactivity Rhesus Monkey
Kits with alternative reactivity to:
(29), (15), (15), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.25-2000 pg/mL
Minimum Detection Limit 31.25 pg/mL
Application ELISA
Supplier Log in to see

Product Details TGFA ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 8.4 pg/mL
Components Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1×

Target details

Product Details TGFA ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TGFa (TGFA ELISA Kit Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details TGFA ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated,Strips (12 x 8)
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Restrictions For Research Use only

Handling

Product Details TGFA ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
Storage 4 °C
Expiry Date 12 months