anti-Rat (Rattus) TNFRSF11A antibody for Western Blotting

Recommended TNFRSF11A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
  • RANK
  • RGD1563614
  • TNFRSF11A
  • Ly109
  • ODFR
  • OFE
  • Rank
  • TRANCE-R
  • mRANK
  • CD265
  • FEO
  • LOH18CR1
  • OPTB7
  • OSTS
  • PDB2
  • TRANCER
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator
  • tumor necrosis factor receptor superfamily, member 11a
  • Tnfrsf11a
  • TNFRSF11A
Epitope
AA 326-616, AA 330-427
20
20
16
15
10
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
116
73
41
Host
Mouse
69
55
9
Clonality
Monoclonal
Conjugate
This TNFRSF11A antibody is un-conjugated
8
5
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
55
35
28
27
13
11
5
5
4
4
4
2
1
Supplier
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Catalog No. ABIN252472
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3477876 ABIN1863084 ICC IHC IP WB Rabbit IgG AA 31-214 Log in to see Polyclonal
3.3477876 ABIN2435278 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN252036 FACS IHC (p) IHC WB PE Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 10
1 ABIN749258 IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal
1 ABIN5518884 IHC (p) WB Rabbit IgG AA 235-262, Middle Region Log in to see Polyclonal
1 ABIN749267 IHC (p) WB HRP Rabbit IgG AA 60-110 Log in to see Polyclonal
1 ABIN749260 IHC (p) WB Biotin Rabbit IgG AA 60-110 Log in to see Polyclonal
1 ABIN4349312 FACS IHC IHC (p) WB PerCP Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349313 FACS IHC IHC (p) WB DyLight 550 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349329 FACS IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349333 FACS IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349327 FACS IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349315 FACS IHC IHC (p) WB DyLight 405 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349301 CyTOF FACS IHC IHC (p) WB Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349306 IHC IHC (p) WB Biotin Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349308 FACS IHC IHC (p) WB DyLight 680 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349314 FACS IHC IHC (p) WB DyLight 350 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349311 FACS IHC IHC (p) WB DyLight 755 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349307 FACS IHC IHC (p) WB DyLight 650 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725
1 ABIN4349309 FACS IHC IHC (p) WB DyLight 488 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725

Top referenced anti-TNFRSF11A antibody for Western Blotting

Similar anti-TNFRSF11A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies 5 Antibodies
ELISA 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 18 Antibodies 45 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 21 Antibodies 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 25 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
Epitope AA 326-616, AA 330-427
(20), (20), (16), (15), (10), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(116), (73), (41)
Host Mouse
(69), (55), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF11A antibody is un-conjugated
(8), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(77), (55), (35), (28), (27), (13), (11), (5), (5), (4), (4), (4), (2), (1)
Pubmed 15 references available
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Product Details anti-TNFRSF11A Antibody

Target Details TNFRSF11A Application Details Handling ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472) Images
Specificity In RAW cells, a 97 kDa is observed.
Purification Protein G purified
Immunogen A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Clone 9A725
Isotype IgG1 kappa

Target Details TNFRSF11A

Product Details anti-TNFRSF11A Antibody Application Details Handling ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472) Images back to top
Antigen
Alternative Name RANK/TNFRSF11A (TNFRSF11A Antibody Abstract)
Background Gene Symbol: TNFRSF11A
Gene ID 8792
UniProt Q9Y6Q6
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Handling ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472) Images back to top
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 2-4 μg/mL, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Cell Surface), CyTOF-readyBharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. Use in Flow cell surface reported in scientific literature (PMID 24376119)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472)

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Handling Images back to top
Product cited in:

Watari, Shibata, Nabeshima, Shinoda, Fukunaga, Kawahara, Karasuyama, Fukushi, Iwamoto, Kuwano, Ono: "Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice." in: Scientific reports, Vol. 6, pp. 19470, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Nanke, Kobashigawa, Yago, Kawamoto, Yamanaka, Kotake: "RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients." in: BioMed research international, Vol. 2016, pp. 4874195, 2016 Method employed by authors: Western Blotting (WB)

Huynh, VonMoss, Smith, Rahman, Felemban, Zuo, Rody, McHugh, Holliday: "Characterization of Regulatory Extracellular Vesicles from Osteoclasts." in: Journal of dental research, Vol. 95, Issue 6, pp. 673-9, 2016 Method employed by authors: Western Blotting (WB)

Kaneko, Ota, Nakashima, Nagasaki, Kodani, Mori, Nagatsu: "Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 122, Issue 2, pp. 187-99, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Das, Sepahi, Duthie, Clark, Crockett: "RANK receptor oligomerisation in the regulation of NFκB signalling." in: Journal of molecular endocrinology, Vol. 53, Issue 1, pp. 81-91, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lafferty, Fantry, Bryant, Jones, Hammoud, Weitzmann, Lewis, Garzino-Demo, Reid: "Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats." in: Pathogens and disease, Vol. 71, Issue 1, pp. 81-9, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Riegel, Maurer, Prior, Stegmaier, Heppert, Wagner, Hänsch: "Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL." in: European journal of immunology, Vol. 42, Issue 4, pp. 975-81, 2012

Izawa, Zou, Chappel, Ashley, Feng, Teitelbaum: "c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton." in: Molecular and cellular biology, Vol. 32, Issue 14, pp. 2943-53, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Cannon, Kraj, Sloan: "Follicle-stimulating hormone promotes RANK expression on human monocytes." in: Cytokine, Vol. 53, Issue 2, pp. 141-4, 2011

Ayon Haro, Ukai, Yokoyama, Kishimoto, Yoshinaga, Hara: "Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation." in: Journal of periodontal research, Vol. 46, Issue 3, pp. 361-73, 2011

Crockett, Mellis, Shennan, Duthie, Greenhorn, Wilkinson, Ralston, Helfrich, Rogers: "Signal peptide mutations in RANK prevent downstream activation of NF-κB." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 26, Issue 8, pp. 1926-38, 2011 Further details: Western Blotting

Huang, Wen, Gao, Zhang, Bai: "An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples." in: Biotechnology letters, Vol. 33, Issue 10, pp. 1939-47, 2011 Further details: Western Blotting

Lee, Kim, Jun, Yoo, Woo, Choi, Lee, Song, Sohn, Park-Min, Ivashkiv, Ji: "Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 10, pp. 5926-34, 2010 (Sample species: Human). Further details: Western Blotting

Bharti, Takada, Shishodia, Aggarwal: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004

Fiumara, Snell, Li, Mukhopadhyay, Younes, Gillenwater, Cabanillas, Aggarwal, Younes: "Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines." in: Blood, Vol. 98, Issue 9, pp. 2784-90, 2001

Images

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Handling ProductDetails: References for anti-TNFRSF11A antibody (ABIN252472) back to top
Supplier Images
Western Blotting (WB) image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Western Blot: RANK Antibody (9A725) [ABIN252472] - Western blot analysis of RANK in 2...
Flow Cytometry (FACS) image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Flow Cytometry: RANK Antibody (9A725) [ABIN252472] - Flow analysis of RANK in RAW cel...
 image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [ABIN2524728] - Analysis using t...