Mouse (Murine) TNFRSF13C ELISA Kit

Recommended TNFRSF13C Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C) ELISA Kits
  • RGD1560810
  • TNFRSF13C
  • BAFF-R
  • BAFFR
  • BROMIX
  • CD268
  • CVID4
  • prolixin
  • 2010006P15Rik
  • Baffr
  • Bcmd
  • Bcmd-1
  • Bcmd1
  • Lvis22
  • tumor necrosis factor receptor superfamily, member 13C
  • tumor necrosis factor receptor superfamily, member 13c
  • tumor necrosis factor receptor superfamily member 13C-like
  • TNFRSF13C
  • Tnfrsf13c
  • LOC618516
Epitope
AA 10-71
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
14
10
4
3
2
2
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1706073
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.76768 ABIN1568577 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 3.125-200 pg/mL 3.125 pg/mL Log in to see
9.76768 ABIN2702844 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 28-7.000 pg/mL 28 pg/mL Log in to see
9.76768 ABIN2708463 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN824994 Sandwich ELISA Serum, Plasma, Tissue Homogenate 10-0.16 ng/mL 10 ng/mL Log in to see
1 ABIN3032796 Competition ELISA Cell Culture Supernatant, Plasma, Serum 28.7-7000 pg/mL 28.7 pg/mL Log in to see
1 ABIN627580 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 10-2500 pg/mL 10 pg/mL Log in to see
1 ABIN5520163 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN2685714 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN4967420 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 3.125-200 pg/mL 3.125 pg/mL Log in to see
1 ABIN1164386 Log in to see

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General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C) ELISA Kits
Epitope AA 10-71
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(14), (10), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details TNFRSF13C ELISA Kit

Target details Application Details Handling ProductDetails: References for TNFRSF13C Kit (ABIN1706073) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF13C/BAFFR
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: S10-A71
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: S10-A71

Target details

Product Details TNFRSF13C ELISA Kit Application Details Handling ProductDetails: References for TNFRSF13C Kit (ABIN1706073) Images back to top
Antigen
Alternative Name TNFRSF13C (TNFRSF13C ELISA Kit Abstract)
Background

Protein Function: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. .

Background: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Synonyms: Tumor necrosis factor receptor superfamily member 13C,B-cell maturation defect,B-cell-activating factor receptor,BAFF receptor,BAFF-R,BLyS receptor 3,CD268,Tnfrsf13c,Baffr, Bcmd, Br3,

Full Gene Name: Tumor necrosis factor receptor superfamily member 13C

Cellular Localisation: Membrane, Single-pass type III membrane protein.
Gene ID 72049
UniProt Q9D8D0
Pathways NF-kappaB Signaling

Application Details

Product Details TNFRSF13C ELISA Kit Target details Handling ProductDetails: References for TNFRSF13C Kit (ABIN1706073) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Highly expressed in spleen and testis, detected at lower levels in lung and thymus.

Plate Pre-coated
Protocol mouse TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NSO, S10-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF13C amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse TNFRSF13C standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF13C standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 562, Standard deviation: 25.3, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 1250, Standard deviation: 42.5, CV(%): 3.4
  • Sample 3: n=16, Mean(pg/ml): 2841, Standard deviation: 142, CV(%): 5,
  • Sample 1: n=24, Mean(pg/ml): 638, Standard deviation: 35.7, CV(%): 5.6
  • Sample 2: n=24, Mean(pg/ml): 1634, Standard deviation: 76.8, CV(%): 4.7
  • Sample 3: n=24, Mean(pg/ml): 3205, Standard deviation: 205.1, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details TNFRSF13C ELISA Kit Target details Application Details ProductDetails: References for TNFRSF13C Kit (ABIN1706073) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for TNFRSF13C Kit (ABIN1706073)

Product Details TNFRSF13C ELISA Kit Target details Application Details Handling Images back to top
Background publications

Thompson, Bixler, Qian, Vora, Scott, Cachero, Hession, Schneider, Sizing, Mullen, Strauch, Zafari, Benjamin, Tschopp, Browning, Ambrose: "BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF." in: Science (New York, N.Y.), Vol. 293, Issue 5537, pp. 2108-11, 2001

Images

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Supplier Images
ELISA image for TNFRSF13C ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 13C) (ABIN1706073) Mouse TNFRSF13C/BAFFR PicoKine ELISA Kit standard curve