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Mouse (Murine) TNFRSF13C ELISA Kit

Recommended TNFRSF13C Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C) ELISA Kits
  • 2010006P15Rik
  • BAFF-R
  • Baffr
  • BAFFR
  • Bcmd
  • Bcmd-1
  • Bcmd1
  • BROMIX
  • CD268
  • CVID4
  • Lvis22
  • prolixin
  • RGD1560810
  • TNFRSF13C
Epitope
AA 10-71
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
13
10
4
3
3
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
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Catalog No. ABIN1706073
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.31849235 ABIN1568577 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 3.125-200 pg/mL 3.125 pg/mL Log in to see
0.31849235 ABIN2708463 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Log in to see
0.0012145293 ABIN824994 Sandwich ELISA Serum, Plasma, Tissue Homogenate 10-0.16 ng/mL 10 ng/mL Log in to see
0.0012145293 ABIN3032796 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 28.7-7000 pg/mL 28.7 pg/mL Log in to see
0.0012145293 ABIN627580 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 10-250 pg/mL 10 pg/mL Log in to see
0.0012145293 ABIN512650 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012145293 ABIN2702844 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 28-7.000 pg/mL 28 pg/mL Log in to see
0.0012145293 ABIN2685714 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 78-5000 pg/mL 78 pg/mL Log in to see
0.0012145293 ABIN1164386 Log in to see
0.0012145293 ABIN2065910 Sandwich ELISA Log in to see

Top referenced TNFRSF13C ELISA Kit for ELISA

Similar TNFRSF13C ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 11 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C) ELISA Kits
Epitope AA 10-71
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(13), (10), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details TNFRSF13C ELISA Kit

Target details Application Details Handling References for TNFRSF13C Kit (ABIN1706073) Images
Purpose For quantitative detection of mouse TNFRSF13C in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse TNFRSF13C
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse TNFRSF13C antibod - 1
  • Lyophilized recombinant mouse TNFRSF13C standar - 10ng/tubex2
  • Biotinylated anti- mouse TNFRSF13C antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: S10-A71

Target details

Product Details TNFRSF13C ELISA Kit Application Details Handling References for TNFRSF13C Kit (ABIN1706073) Images back to top
Antigen
Alternative Name TNFRSF13C (TNFRSF13C ELISA Kit Abstract)
Background Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Gene ID 72049
UniProt Q9D8D0
Pathways NF-kappaB Signaling

Application Details

Product Details TNFRSF13C ELISA Kit Target details Handling References for TNFRSF13C Kit (ABIN1706073) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NSO, S10-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF13C amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse TNFRSF13C standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF13C standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 562, Standard deviation: 25.3, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 1250, Standard deviation: 42.5, CV(%): 3.4
  • Sample 3: n=16, Mean(pg/ml): 2841, Standard deviation: 142, CV(%): 5,
  • Sample 1: n=24, Mean(pg/ml): 638, Standard deviation: 35.7, CV(%): 5.6
  • Sample 2: n=24, Mean(pg/ml): 1634, Standard deviation: 76.8, CV(%): 4.7
  • Sample 3: n=24, Mean(pg/ml): 3205, Standard deviation: 205.1, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details TNFRSF13C ELISA Kit Target details Application Details References for TNFRSF13C Kit (ABIN1706073) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFRSF13C Kit (ABIN1706073)

Product Details TNFRSF13C ELISA Kit Target details Application Details Handling Images back to top
Background publications

Thompson, Bixler, Qian, Vora, Scott, Cachero, Hession, Schneider, Sizing, Mullen, Strauch, Zafari, Benjamin, Tschopp, Browning, Ambrose: "BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF." in: Science (New York, N.Y.), Vol. 293, Issue 5537, pp. 2108-11, 2001

Images

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Supplier Images
ELISA image for TNFRSF13C ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 13C) (ABIN1706073) Mouse TNFRSF13C/BAFFR PicoKine ELISA Kit standard curve