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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||34 Antibodies|
|Functional Studies (Func)||8 Antibodies|
|Immunoassay (IA)||3 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||21 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||45 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||62 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Neutralization (Neut)||4 Antibodies|
|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TNFRSF1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TNFRSF1A AntibodyTarget Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN358242) Images
|Specificity||This antibody detects TNFR pSer274.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S274 of human TNFR.|
Target Details TNFRSF1AProduct Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A Antibody (ABIN358242) Images back to top
|Alternative Name||CD120a / TNFR1 (TNFRSF1A Antibody Abstract)|
|Background||A member of the TNF-receptor superfamily, this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60|
|Molecular Weight||50495 Da|
|Gene ID||7132, 9606|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A Antibody (ABIN358242) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A Antibody (ABIN358242) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-TNFRSF1A Antibody (ABIN358242)Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
Kuo, Lympany, Menezo et al.: "TNF-857T, a genetic risk marker for acute anterior uveitis." in: Investigative ophthalmology & visual science, Vol. 46, Issue 5, pp. 1565-71, 2005 (PubMed).
Siebert, Amos, Fielding et al.: "Reduced tumor necrosis factor signaling in primary human fibroblasts containing a tumor necrosis factor receptor superfamily 1A mutant." in: Arthritis and rheumatism, Vol. 52, Issue 4, pp. 1287-92, 2005 (PubMed).
Wang, Grégori, Hullinger et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor ..." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).
Tashiro, Itamoto, Ohdan et al.: "Involvement of tumor necrosis factor-alpha receptor 1 and tumor necrosis factor-related apoptosis-inducing ligand-(TRAIL) receptor-2/DR-5, but not Fas, in graft injury in live-donor liver transplantation." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 17, Issue 10, pp. 626-33, 2004 (PubMed).
Spahr, Giostra, Frossard et al.: "Soluble TNF-R1, but not tumor necrosis factor alpha, predicts the 3-month mortality in patients with alcoholic hepatitis." in: Journal of hepatology, Vol. 41, Issue 2, pp. 229-34, 2004 (PubMed).
ImagesProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN358242) back to top