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anti-Mouse (Murine) TNFRSF1A antibody for Flow Cytometry

Recommended TNFRSF1A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
  • cd120a
  • CD120a
  • fpf
  • FPF
  • MS5
  • p55
  • p55-R
  • p55-r
  • p60
  • TBP1
  • tbp1
  • TNF-alphaR1
  • TNF-R
  • tnf-r
  • TNF-R-I
  • TNF-R1
  • TNF-R55
  • TNFalpha-R1
  • tnfar
  • TNFAR
  • Tnfr-2
  • tnfr1
  • Tnfr1
  • TNFR1
  • TNFR1-d2
  • tnfr55
  • TNFR55
  • TNFR60
  • tnfr60
  • TNFR I
  • TNFRI
  • TNFRp55
  • TNFRSF1A
  • wu:fj65b10
  • xtnfr1
  • zgc:85914
Reactivity
Mouse (Murine)
324
161
81
38
33
31
27
14
9
8
8
7
6
4
4
2
2
1
1
Host
Armenian Hamster
257
110
17
17
16
14
1
Clonality
Monoclonal
Conjugate
This TNFRSF1A antibody is un-conjugated
32
30
29
13
10
9
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
259
166
149
113
102
67
53
34
31
23
22
12
11
10
7
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN967653
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30391768 ABIN2660923 FACS CyTOF Biotin Hamster IgG Log in to see 55R-170 5
0.30391768 ABIN2658462 FACS APC Hamster IgG Log in to see 55R-286 10
0.30391768 ABIN2662979 FACS IHC PE Hamster IgG Log in to see 55R-286 10
0.30391768 ABIN2665528 FACS Hamster IgG Log in to see 55R-593 5
0.30391768 ABIN2664174 FACS Hamster IgG Log in to see 55R-286 9
0.0010469793 ABIN1105797 EIA IHC (fro) FACS FITC Rat IgG2a Log in to see HM104 3
0.0010469793 ABIN1105791 EIA IHC (fro) FACS IP Rat IgG2a Log in to see HM104 3
0.0010469793 ABIN114263 FACS PE Rat IgG2a Log in to see HM104
0.0010469793 ABIN114262 FACS PE Rat IgG2a Log in to see HM104
0.0010469793 ABIN1830498 Func FACS IP ELISA FITC Hamster IgG1 Extracellular Domain Log in to see 13A86
0.0010469793 ABIN602487 IHC (fro) FACS IP ELISA Rat IgG2a Log in to see HM14
0.0010469793 ABIN2660922 FACS CyTOF Biotin Hamster IgG Log in to see 55R-170 5
0.0010469793 ABIN2658463 FACS APC Hamster IgG Log in to see 55R-286 10
0.0010469793 ABIN2662980 FACS IHC PE Hamster IgG Log in to see 55R-286 10
0.0010469793 ABIN602491 IHC (fro) FACS IHC (p) FITC Rat IgG2a Log in to see
0.0010469793 ABIN319506 FACS PE Rat IgG2a Log in to see HM104
0.0010469793 ABIN259883 FACS IP ELISA WB Armenian Hamster IgG Extracellular Domain Log in to see RM0011-6F13
0.0010469793 ABIN134005 FACS PE Rat IgG2a Log in to see
0.0010469793 ABIN959777 IHC (fro) FACS IP ELISA Rat IgG2a Log in to see HM14
0.0010469793 ABIN2377489 FACS IP ELISA FITC Hamster IgG1 Log in to see 13A86

Top referenced anti-TNFRSF1A antibody for Flow Cytometry

Similar anti-TNFRSF1A Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 77 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Neutralization (Neut) 4 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
Reactivity Mouse (Murine)
(324), (161), (81), (38), (33), (31), (27), (14), (9), (8), (8), (7), (6), (4), (4), (2), (2), (1), (1)
Host Armenian Hamster
(257), (110), (17), (17), (16), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1A antibody is un-conjugated
(32), (30), (29), (13), (10), (9), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(259), (166), (149), (113), (102), (67), (53), (34), (31), (23), (22), (12), (11), (10), (7), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-TNFRSF1A Antibody

Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN967653) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse TNFRI
Clone 55R-286
Isotype IgG1, kappa

Target Details TNFRSF1A

Product Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A Antibody (ABIN967653) Images back to top
Antigen
Alternative Name CD120a (TNFRSF1A Antibody Abstract)
Background The 55R-286 antibody reacts with the extracellular region of the 55 kDa receptor for the mouse cytokines, Tumor Necrosis Factor (TNF) and lymphotoxin-alpha (LT-alpha). This receptor, referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI) [aka, CD120a], is expressed by a variety of cell lines and normal cell types including T cells, monocytes, macrophages, and neutrophils. B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes are uniformly negative for TNFRI expression. In addition, the 55R-286 antibody can bind to a soluble, truncated form of the mouse Type I TNFR that is shed by cells in response to certain stimuli, e.g., cells treated with LPS or TNF. The in vivo administration of nonblocking, nonagonistic 55R-286 antibody reportedly results in the linear accumulation of shed, soluble forms of the TNFRI in the circulation. The 55R-286 antibody does not recognize the 75 kD (p75) Type II TNFR (aka, CD120b). 55R-286 does not block the binding and does not neutralize the bioactivity of the TNF ligand on L929 target cell populations that express TNFRI. The immunogen used to generate the 55R-286 hybridoma was a purified, soluble extracellular domain of the mouse TNFRI.
Synonyms: TNF Receptor, Type I/p55
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A Antibody (ABIN967653) Images back to top
Application Notes 1) ELISA Capture:
The purified 55R-286 antibody (ABIN967653) is useful as a capture antibody for a sandwich ELISA for measuring mouse TNFRI protein levels. Purified 55R-286 antibody can be paired with the biotinylated 55R-170 hamster anti-mouse TNFRI as the detecting antibody. Purified 55R-286 antibody should be titrated 4 - 8 µg/ml to determine its optimal concentration for ELISA capture.
Note: This ELISA antibody pair shows no cross-reactivity with other purified recombinant cytokines or receptors that were tested including:
1) Mouse IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12 p40, IL-12p70, IL-16, IL-17, GM-CSF, IFN-gamma, TNF, TNFRI, TNFRII, MCP-1, CRG-2,
2) Human IL-1alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-16, GM-CSF, G-CSF, TNF, LT-alpha, TNFRI, TNFRII, MCP-1, MCP-2, IFN-gamma, RANTES, MIG
3) Rat IL-1alpha, IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, MCP-1, TNF

2) Immunofluorescent Staining and Flow Cytometric Analysis:
The purified form of 55R-286 (ABIN967653) can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10^6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of TNFRI. An appropriate purified isotype control is clone A19-3. A three-layer staining protocol is recommended for maximizing the detection of TNFRI expressed by cells as detailed in the figure legend.
Note: 55R-286 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where ligands (eg, TNF) for TNF receptors are present. Based on our testing results (data not shown), the presence of exogenous recombinant mouse TNF at levels Less or equal than 1 µg per 10^6 cells was insufficient to inhibit the binding of 55R-286 to L929 cells that express p55 TNFR (at 0.25 µg antibody/10^6 cells). Please note also that as a consequence of in vivo or in vitro activation, cell surface TNFRI can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cells overall expressed level of surface TNFRI.

Immunoprecipitation:
The 55R-286 antibody has been reported to be useful for the immunoprecipitation of p55 TNFR from lysates of mouse Meth A fibrosarcoma cells. Please note that this application is not routinely tested.

WB:
The purified 55R-286 antibody has been found useful for Western blotting. Please note that this application is not routinely tested.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A Antibody (ABIN967653) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-TNFRSF1A Antibody (ABIN967653)

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
Product cited in:

Pinckard, Sheehan, Schreiber: "Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10784-9, 1997

Pinckard, Sheehan, Arthur, Schreiber: "Constitutive shedding of both p55 and p75 murine TNF receptors in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 8, pp. 3869-73, 1997

Sheehan, Pinckard, Arthur, Dehner, Goeddel, Schreiber: "Monoclonal antibodies specific for murine p55 and p75 tumor necrosis factor receptors: identification of a novel in vivo role for p75." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 607-17, 1995

Images

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN967653) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-TNFRSF1A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 1A) (ABIN967653) Overlapping Histograms Figure: Expression of cell surface TNFRI by BALB/c lymph node ...
 image for anti-TNFRSF1A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 1A) (ABIN967653) Histogram: FITC-B220-positive