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anti-Human TNFRSF1B antibody for Functional Studies

Recommended TNFRSF1B Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
  • CD120b
  • LOC100218184
  • MGC83471
  • p75
  • p75TNFR
  • TANK
  • TBPII
  • TNF-alphaR2
  • TNF-R-II
  • TNF-R2
  • TNF-R75
  • TNF-RII/TNFRSF1B
  • TNFalpha-R2
  • TNFBR
  • Tnfr-1
  • TNFR-II
  • TNFR1B
  • Tnfr2
  • TNFR2
  • TNFR80
  • TNFRII
  • TNFRSF1B
  • TRAF2
Reactivity
Human
143
84
34
3
1
Host
Mouse
117
54
13
12
7
3
1
Clonality
Monoclonal
Conjugate
This TNFRSF1B antibody is un-conjugated
27
12
7
4
2
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
137
79
57
46
30
29
26
26
19
19
9
9
4
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN111942
$ 1,424.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008738719 ABIN1105817 Func EIA FACS IF IP FITC Mouse IgG1 Log in to see 80M2 5
0.0008738719 ABIN1105810 Func EIA FACS IF IP Mouse IgG1 Log in to see 80M2 5
0.0008738719 ABIN1105809 Func EIA IHC (fro) FACS IP WB Mouse IgG1 Log in to see MR2-1 3
0.0008738719 ABIN180971 EIA Func IHC (fro) WB Mouse IgG2a Log in to see 31-01-20
0.0008738719 ABIN1105811 Func EIA IF IP Biotin Mouse IgG1 Log in to see 80M2 5
0.0008738719 ABIN1105815 Func EIA IHC (fro) IP WB FITC Mouse IgG1 Log in to see MR2-1 3
0.0008738719 ABIN198261 Func IHC (fro) ELISA WB Mouse IgG2a Log in to see 2-220
0.0008738719 ABIN255990 Func IHC (fro) IHC ELISA WB Mouse IgG2a Log in to see 2-220
0.0008738719 ABIN1105808 Func EIA FACS IP WB Rabbit IgG Log in to see Polyclonal
0.0008738719 ABIN2191888 Func IA FACS FITC Mouse IgG1 Log in to see 80M2 3
0.0008738719 ABIN2191889 Func IA FACS Biotin Mouse IgG1 Log in to see 80M2 3
0.0008738719 ABIN602495 Func IHC (fro) IHC (p) ELISA WB Mouse IgG2a Log in to see
0.0008738719 ABIN2191887 Func IA FACS Mouse IgG1 Log in to see 80M2 3
0.0008738719 ABIN2192216 Func IA FACS IP WB FITC Mouse Log in to see MR2-1 1
0.0008738719 ABIN640532 Func IHC (fro) ELISA WB Mouse IgG2a Log in to see
0.0008738719 ABIN2478300 Func IHC (fro) ELISA WB Mouse IgG2a Log in to see 2-220
0.0008738719 ABIN2478301 Func IF ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-TNFRSF1B antibody for Functional Studies

Similar anti-TNFRSF1B Antibodies

Application / Reactivity Human
Agonist (Agon) 1 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Cell Culture (CC) 2 Antibodies
ELISA 55 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 39 Antibodies
Functional Studies (Func) 18 Antibodies
Immunoassay (IA) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 98 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
Reactivity Human
(143), (84), (34), (3), (1)
Host Mouse
(117), (54), (13), (12), (7), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1B antibody is un-conjugated
(27), (12), (7), (4), (2), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(137), (79), (57), (46), (30), (29), (26), (26), (19), (19), (9), (9), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-TNFRSF1B Antibody

Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B Antibody (ABIN111942) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen Partially purified preparations of TNF binding proteins from U937 cells.
Clone Utr1
Isotype IgG1

Target Details TNFRSF1B

Product Details anti-TNFRSF1B Antibody Application Details Handling References for anti-TNFRSF1B Antibody (ABIN111942) Images back to top
Antigen
Alternative Name CD120b / TNFR2 (TNFRSF1B Antibody Abstract)
Background Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
Gene ID 9606
UniProt P20333
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Apoptosis

Application Details

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Handling References for anti-TNFRSF1B Antibody (ABIN111942) Images back to top
Application Notes Immunohistology: 2-5 μg/mL on Cryostat Sections (2 % Periodate-lysine-paraformaldehydeor acetone for fixation). Flow Cytometry: 10 μg/mL. Immunoprecipitation. Western blots: 30 μg/mL on non-reduced SDS PAGE, the epitope is sensitive to reduction,may need enrichment by Immunoprecipitation. Functional Assay: This antibody utr1 is useful for studying biological effects ofTNF-Receptor p75 in vitro where it inhibits the binding of radiolabelled TNF to human cellsexpressing the p75 TNF receptor. In order to completely block TNF binding to the cellsurface, 10 μg/mL of utr1 are required. utr1 itself may have an agonistic effect in assaysmeasuring cytotoxicity, fibroblast growth or IL-6 secretion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details References for anti-TNFRSF1B Antibody (ABIN111942) Images back to top
Concentration 2.0 mg/mL (by Absorbanece at 280 nm)
Buffer PBS, pH 7.2, None, None
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

References for anti-TNFRSF1B Antibody (ABIN111942)

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling Images back to top
Background publications

Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, 1992

Vandenabeele, Declercq, Vercammen, Van de Craen, Grooten, Loetscher, Brockhaus, Lesslauer, Fiers: "Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1015-24, 1992

Ryffel, Brockhaus, Dürmüller, Gudat: "Tumor necrosis factor receptors in lymphoid tissues and lymphomas. Source and site of action of tumor necrosis factor alpha." in: The American journal of pathology, Vol. 139, Issue 1, pp. 7-15, 1991

Hohmann, Brockhaus, Baeuerle, Remy, Kolbeck, van Loon: "Expression of the types A and B tumor necrosis factor (TNF) receptors is independently regulated, and both receptors mediate activation of the transcription factor NF-kappa B. TNF alpha is not needed for induction of a biological effect via TNF receptors." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22409-17, 1991

Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, 1990

Espevik, Brockhaus, Loetscher, Nonstad, Shalaby: "Characterization of binding and biological effects of monoclonal antibodies against a human tumor necrosis factor receptor." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 415-26, 1990

Shalaby, Sundan, Loetscher, Brockhaus, Lesslauer, Espevik: "Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors." in: The Journal of experimental medicine, Vol. 172, Issue 5, pp. 1517-20, 1990