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|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies|
|Conjugate||This TNFRSF1B antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
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Product Details anti-TNFRSF1B AntibodyTarget Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B antibody (ABIN111940) Images
|Immunogen||Partially purified TNF binding proteins|
Target Details TNFRSF1BProduct Details anti-TNFRSF1B Antibody Application Details Handling References for anti-TNFRSF1B antibody (ABIN111940) Images back to top
|Alternative Name||CD120b / TNFR2 (TNFRSF1B Antibody Abstract)|
|Background||Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins of 55 and 75 kDa respectively. They are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C|
Application DetailsProduct Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Handling References for anti-TNFRSF1B antibody (ABIN111940) Images back to top
Immunohistochemistry on Frozen Sections: 5 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: 50 μg/mL (1/20). Pretreatment not necessary. Has been described to work in FACS, Immunoprecipitation and Western Blots. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature|
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details References for anti-TNFRSF1B antibody (ABIN111940) Images back to top
|Reconstitution||Restore with 0.5 mL distilled water|
|Buffer||PBS, pH 7.2, 0.05 % Kathon CG, 5 mg/mL BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-TNFRSF1B antibody (ABIN111940)Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling Images back to top
Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, 1992
Vandenabeele, Declercq, Vercammen, Van de Craen, Grooten, Loetscher, Brockhaus, Lesslauer, Fiers: "Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1015-24, 1992
Ryffel, Brockhaus, Dürmüller, Gudat: "Tumor necrosis factor receptors in lymphoid tissues and lymphomas. Source and site of action of tumor necrosis factor alpha." in: The American journal of pathology, Vol. 139, Issue 1, pp. 7-15, 1991
Hohmann, Brockhaus, Baeuerle, Remy, Kolbeck, van Loon: "Expression of the types A and B tumor necrosis factor (TNF) receptors is independently regulated, and both receptors mediate activation of the transcription factor NF-kappa B. TNF alpha is not needed for induction of a biological effect via TNF receptors." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22409-17, 1991
Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, 1990
Espevik, Brockhaus, Loetscher, Nonstad, Shalaby: "Characterization of binding and biological effects of monoclonal antibodies against a human tumor necrosis factor receptor." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 415-26, 1990
Shalaby, Sundan, Loetscher, Brockhaus, Lesslauer, Espevik: "Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors." in: The Journal of experimental medicine, Vol. 172, Issue 5, pp. 1517-20, 1990