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anti-Mouse (Murine) TNFRSF1B antibody for Flow Cytometry

Recommended TNFRSF1B Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
  • CD120b
  • LOC100218184
  • MGC83471
  • p75
  • p75TNFR
  • TANK
  • TBPII
  • TNF-alphaR2
  • TNF-R-II
  • TNF-R2
  • TNF-R75
  • TNF-RII/TNFRSF1B
  • TNFalpha-R2
  • TNFBR
  • Tnfr-1
  • TNFR-II
  • TNFR1B
  • Tnfr2
  • TNFR2
  • TNFR80
  • TNFRII
  • TNFRSF1B
  • TRAF2
Reactivity
Mouse (Murine)
144
83
34
3
1
Host
Armenian Hamster
117
55
13
12
7
2
1
Clonality
Monoclonal
Conjugate
This TNFRSF1B antibody is un-conjugated
27
12
7
4
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
138
79
57
46
30
30
27
26
19
19
9
9
4
2
2
2
1
1
1
1
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Catalog No. ABIN967654
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN2660924 FACS IHC ELISA Biotin Hamster IgG Log in to see TR75-89 7
0.3068567 ABIN2660925 Func FACS IHC ELISA Biotin Hamster IgG Log in to see TR75-32-4 5
0.3068567 ABIN2665530 FACS Hamster IgG Log in to see TR75-54-7 5
0.3068567 ABIN2665529 FACS ICFC Hamster IgG Log in to see TR75-32-4 6
0.3068567 ABIN2191751 Func IA FACS IP WB FITC Rat Log in to see HM102 2
0.3068567 ABIN2191752 Func IA FACS IP WB Biotin Rat Log in to see HM102 2
0.3068567 ABIN2191750 Func IA FACS IP WB Rat Log in to see HM102 2
0.0010666476 ABIN1105801 EIA IHC (fro) FACS IP Rat IgG2a Log in to see HM102 2
0.0010666476 ABIN1032953 Func FACS IP ELISA WB Armenian Hamster IgG Log in to see TR75-54 2
0.0010666476 ABIN2688928 FACS Biotin Armenian Hamster IgG1, lambda Log in to see TR75 1
0.0010666476 ABIN602493 IHC (fro) FACS ELISA FITC Rat IgG2a Log in to see
0.0010666476 ABIN602497 IHC (fro) FACS IP ELISA Rat IgG2a Log in to see
0.0010666476 ABIN259884 FACS IP ELISA WB Armenian Hamster IgG Extracellular Domain Log in to see RM0012-6F13
0.0010666476 ABIN1889572 FACS WB Hamster IgG AA 23-258 Log in to see RM0012-6F13
0.0010666476 ABIN215526 FACS IHC (fro) IP Biotin Rat IgG2a Log in to see
0.0010666476 ABIN1838193 IHC (fro) FACS IP Rat IgG2a Log in to see HM102
0.0010666476 ABIN2192162 Func IA FACS IP WB Rabbit IgG Log in to see Polyclonal 3
0.0010666476 ABIN2988693 ICC FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010666476 ABIN2693139 FACS APC Rabbit Log in to see
0.0010666476 ABIN2693141 FACS PE Rabbit Log in to see

Top referenced anti-TNFRSF1B antibody for Flow Cytometry

Similar anti-TNFRSF1B Antibodies

Application / Reactivity Mouse (Murine)
ELISA 30 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Functional Studies (Func) 10 Antibodies
Immunoassay (IA) 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 61 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies
Reactivity Mouse (Murine)
(144), (83), (34), (3), (1)
Host Armenian Hamster
(117), (55), (13), (12), (7), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1B antibody is un-conjugated
(27), (12), (7), (4), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(138), (79), (57), (46), (30), (30), (27), (26), (19), (19), (9), (9), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-TNFRSF1B Antibody

Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B Antibody (ABIN967654) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Type II TNFR
Clone TR75-89
Isotype IgG1, lambda

Target Details TNFRSF1B

Product Details anti-TNFRSF1B Antibody Application Details Handling References for anti-TNFRSF1B Antibody (ABIN967654) Images back to top
Antigen
Alternative Name CD120b (TNFRSF1B Antibody Abstract)
Background The TR75-89 antibody reacts with the extracellular region of CD120b, the 75 kDa receptor for the mouse cytokines, tumor necrosis factor (TNF, aka TNF-alpha) and lymphotoxin-alpha (LT-alpha, aka lymphotoxin, TNF-beta). This receptor, referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII), or TNFRSF1B, is expressed by a variety of cell lines and normal cell types including T cells, monocytes, macrophages, and neutrophils. Resting B cells express low or undetectable levels of TNFRII whereas mature erythrocytes are uniformly negative for TNFRII expression. In addition, the TR75-89 antibody can bind to a soluble, truncated form of the mouse Type II TNFR that is shed by cells in response to certain stimuli, e.g., cells treated with LPS or TNF. The in vivo administration of nonblocking, nonagonistic TR75-89 antibody reportedly results in the linear accumulation of shed, soluble forms of p75 TNFR in the circulation. The TR75-89 antibody does not recognize the 55 kDa (p55) Type I TNFR (aka, CD120a). TR75-89 does not block the binding and does not neutralize the bioactivity of the TNF ligand on L929 target cell populations that express p75 (and p55) TNFR. The immunogen used to generate the TR75-89 hybridoma was a purified, soluble extracellular domain of the mouse Type II TNFR. This antibody is routinely tested by flow cytometric analysis.
Synonyms: TNF Receptor, Type II/p75
Pathways NF-kappaB Signaling, Apoptosis

Application Details

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Handling References for anti-TNFRSF1B Antibody (ABIN967654) Images back to top
Application Notes Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of TR75-89 (ABIN967654) can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of p75 TNFR. An appropriate purified immunoglobulin isotype control is clone G235-2356. A three-layer staining protocol is recommended for maximizing the detection of p75 TNFR expressed by cells as detailed in the figure legend.
TR75-89 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where ligands (e.g., TNF) for p75 TNF receptors are present. Based on our testing results (data not shown), the presence of exogenous recombinant mouse TNF at levels Less or equal than 1 µg per 10e6 cells was insufficient to inhibit the binding of TR75-89 to L929 cells that express p75 TNFR (at 0.25 µg antibody/10e6 cells). Please note also that as a consequence of in vivo or in vitro activation, cell surface p75 TNFR can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cell's overall expressed level of surface p75 TNFR. IP: The TR75-89 antibody has been reported to be useful for the immunoprecipitation of p75 TNFR from lysates of mouse Meth A fibrosarcoma cells. Please note that this application is not routinely tested.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details References for anti-TNFRSF1B Antibody (ABIN967654) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.

References for anti-TNFRSF1B Antibody (ABIN967654)

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling Images back to top
Product cited in:

Zola: "Detection of cytokine receptors by flow cytometry." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.21, 2008

Pinckard, Sheehan, Arthur, Schreiber: "Constitutive shedding of both p55 and p75 murine TNF receptors in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 8, pp. 3869-73, 1997

Pinckard, Sheehan, Schreiber: "Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10784-9, 1997

Sheehan, Pinckard, Arthur, Dehner, Goeddel, Schreiber: "Monoclonal antibodies specific for murine p55 and p75 tumor necrosis factor receptors: identification of a novel in vivo role for p75." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 607-17, 1995

Images

Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B Antibody (ABIN967654) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-TNFRSF1B antibody (Tumor Necrosis Factor Receptor Superfamily, Member 1B) (ABIN967654) Expression of cell surface p75 TNFR by BALB/c lymph node T cells. BALB/c lymph node c...