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Mouse (Murine) TNFRSF1B ELISA Kit

Recommended TNFRSF1B Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kits
  • CD120b
  • LOC100218184
  • MGC83471
  • p75
  • p75TNFR
  • TANK
  • TBPII
  • TNF-alphaR2
  • TNF-R-II
  • TNF-R2
  • TNF-R75
  • TNF-RII/TNFRSF1B
  • TNFalpha-R2
  • TNFBR
  • Tnfr-1
  • TNFR-II
  • TNFR1B
  • Tnfr2
  • TNFR2
  • TNFR80
  • TNFRII
  • TNFRSF1B
  • TRAF2
Epitope
AA 23-258
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
18
11
7
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889302
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN924869 Sandwich ELISA Cell Lysate, Serum, Plasma 8-500 pg/mL 8 pg/mL Log in to see
0.30628014 ABIN1117668 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.30628014 ABIN2708441 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN367718 Sandwich ELISA Serum, Plasma, Tissue Homogenate 7.8125-500 pg/mL 7.8125 pg/mL Log in to see
0.0012155735 ABIN2115115 Cell Lysate, Serum, Plasma 8-500 pg/mL 8 pg/mL Log in to see
0.0012155735 ABIN2685727 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2762478 Serum, Plasma, Tissue Homogenate 7.8125-500 pg/mL 7.8125 pg/mL Log in to see
0.0012155735 ABIN2685726 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012155735 ABIN456709 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2952177 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2691151 Log in to see

Top referenced TNFRSF1B ELISA Kit for ELISA

Similar TNFRSF1B ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 12 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kits
Epitope AA 23-258
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(18), (11), (7), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TNFRSF1B ELISA Kit

Target details Application Details Handling References for TNFRSF1B Kit (ABIN1889302) Images
Purpose For quantitative detection of mouse TNFR2 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse TNFR2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- mouse TNFR2 antibod - 1
  • Lyophilized recombinant mouse TNFR2 standar - 10ng/tubex2
  • Biotinylated anti- mouse TNFR2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: V23-G258

Target details

Product Details TNFRSF1B ELISA Kit Application Details Handling References for TNFRSF1B Kit (ABIN1889302) Images back to top
Antigen
Alternative Name TNFRSF1B (TNFRSF1B ELISA Kit Abstract)
Background Tumor necrosis factor receptor 2(TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors(TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55- kDa protein, and TNFR2, a 70- kDa protein, have been mapped to human chromosomes 12(12pter-cen) and 1(1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.
Gene ID 21938
UniProt P25119
Pathways NF-kappaB Signaling, Apoptosis

Application Details

Product Details TNFRSF1B ELISA Kit Target details Handling References for TNFRSF1B Kit (ABIN1889302) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TNFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR2 has been precoated onto 96-well plates. Standards(NSO, V23-G258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFR2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 92, Standard deviation: 4.6, CV(%): 5
  • Sample 2: n=16, Mean(pg/ml): 263, Standard deviation: 11.57, CV(%): 4.4
  • Sample 3: n=16, Mean(pg/ml): 492, Standard deviation: 27.6, CV(%): 5.6,
  • Sample 1: n=24, Mean(pg/ml): 104, Standard deviation: 5.4, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 253, Standard deviation: 14.67, CV(%): 5.8
  • Sample 3: n=24, Mean(pg/ml): 527, Standard deviation: 32.2, CV(%): 6.1
Restrictions For Research Use only

Handling

Product Details TNFRSF1B ELISA Kit Target details Application Details References for TNFRSF1B Kit (ABIN1889302) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFRSF1B Kit (ABIN1889302)

Product Details TNFRSF1B ELISA Kit Target details Application Details Handling Images back to top
Background publications

Vielhauer, Stavrakis, Mayadas: "Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis." in: The Journal of clinical investigation, Vol. 115, Issue 5, pp. 1199-209, 2005

Milatovich, Song, Heller, Francke: "Tumor necrosis factor receptor genes, TNFR1 and TNFR2, on human chromosomes 12 and 1." in: Somatic cell and molecular genetics, Vol. 17, Issue 5, pp. 519-23, 1992

Images

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Supplier Images
ELISA image for TNFRSF1B ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 1B) (ABIN1889302) Mouse TNFR2 PicoKine ELISA Kit standard curve