anti-Human TNFSF11 antibody for Enzyme Immunoassay

Recommended TNFSF11 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
  • TNFSF11
  • BTF3L1
  • HUMBTFB
  • OCIF
  • OPG
  • TNFRSF11B
  • CD254
  • ODF
  • OPGL
  • OPTB2
  • RANKL
  • TRANCE
  • hRANKL2
  • sOdf
  • Ly109l
  • Trance
  • Opg
  • tumor necrosis factor (ligand) superfamily, member 11
  • basic transcription factor 3 pseudogene 11
  • tumor necrosis factor receptor superfamily, member 11b
  • TNFSF11
  • BTF3P11
  • Tnfsf11
  • Tnfrsf11b
  • TNFRSF11B
Reactivity
Human
241
179
77
3
1
1
1
Host
Rabbit
187
90
37
8
1
Clonality
Polyclonal
Conjugate
This TNFSF11 antibody is un-conjugated
27
12
9
8
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
241
113
92
73
50
37
26
25
23
14
13
7
5
4
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN116281
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116282 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 2
1 ABIN5555262 EIA IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN116279 EIA WB Biotin Rabbit Log in to see Polyclonal 1
1 ABIN116280 EIA WB Biotin Rabbit Log in to see Polyclonal 1
1 ABIN187451 EIA WB Rat IgG1 Log in to see Ranky-1
1 ABIN115783 EIA Mouse IgG1 Log in to see
1 ABIN272132 EIA IF WB Rabbit Log in to see Polyclonal
1 ABIN181556 EIA WB Goat IgG Log in to see Polyclonal
1 ABIN181553 EIA WB Biotin Goat Log in to see Polyclonal
1 ABIN5540147 EIA Rabbit IgG AA 287-304 Log in to see Polyclonal
1 ABIN5540146 EIA Rabbit IgG AA 287-304 Log in to see Polyclonal
1 ABIN5540148 EIA Rabbit AA 287-304 Log in to see Polyclonal
1 ABIN5540149 EIA Rabbit AA 287-304 Log in to see Polyclonal

Top referenced anti-TNFSF11 antibody for Enzyme Immunoassay

Similar anti-TNFSF11 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 4 Antibodies
Cell Culture (CC) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
ELISA 86 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 14 Antibodies
Flow Cytometry (FACS) 58 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 36 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
Reactivity Human
(241), (179), (77), (3), (1), (1), (1)
Host Rabbit
(187), (90), (37), (8), (1)
Clonality
Polyclonal
Conjugate This TNFSF11 antibody is un-conjugated
(27), (12), (9), (8), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(241), (113), (92), (73), (50), (37), (26), (25), (23), (14), (13), (7), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-TNFSF11 Antibody

Target Details TNFSF11 Application Details Handling ProductDetails: References for anti-TNFSF11 antibody (ABIN116281) Images
Specificity This antibody reacts with soluble RANK Ligand.
Purification Affinity Chromatography.
Immunogen Highly pure (>98%) recombinant Human sRANKL.

Target Details TNFSF11

Product Details anti-TNFSF11 Antibody Application Details Handling ProductDetails: References for anti-TNFSF11 antibody (ABIN116281) Images back to top
Antigen
Alternative Name CD254 / RANKL (TNFSF11 Antibody Abstract)
Background RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
Gene ID 8600
NCBI Accession NP_003692
UniProt O14788
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Handling ProductDetails: References for anti-TNFSF11 antibody (ABIN116281) Images back to top
Application Notes Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 μg/mL withan overnight incubation at 4 °C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary
Restrictions For Research Use only

Handling

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details ProductDetails: References for anti-TNFSF11 antibody (ABIN116281) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

ProductDetails: References for anti-TNFSF11 antibody (ABIN116281)

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Handling Images back to top
Background publications

Grimaud, Soubigou, Couillaud, Coipeau, Moreau, Passuti, Gouin, Redini, Heymann: "Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis." in: The American journal of pathology, Vol. 163, Issue 5, pp. 2021-31, 2003