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anti-Human TNFSF11 antibody for Western Blotting

Recommended TNFSF11 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
  • BTF3L1
  • CD254
  • hRANKL2
  • HUMBTFB
  • Ly109l
  • OCIF
  • ODF
  • OPG
  • Opg
  • OPGL
  • OPTB2
  • RANKL
  • sOdf
  • TNFRSF11B
  • TNFSF11
  • TRANCE
  • Trance
Epitope
Center
15
14
13
13
11
10
9
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
169
116
39
1
1
1
1
Host
Rabbit
163
47
29
7
1
Clonality
Polyclonal
Conjugate
This TNFSF11 antibody is un-conjugated
26
12
6
6
5
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
173
107
46
44
28
22
21
12
10
10
9
5
5
3
1
1
1
1
Supplier
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Catalog No. ABIN500805
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12445667 ABIN1533487 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.12445667 ABIN2668631 WB Mouse IgG1 AA 1-317 Log in to see 12A380 1
0.12445667 ABIN2856613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.12445667 ABIN2856614 WB Rabbit IgG N-Term Log in to see Polyclonal
0.12445667 ABIN652842 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal
0.12445667 ABIN2705774 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.12445667 ABIN1875141 IHC WB Rabbit IgG Log in to see Polyclonal
0.12445667 ABIN2500485 IF ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12445667 ABIN1860402 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 79-247 Log in to see Polyclonal
0.12445667 ABIN3187907 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.12445667 ABIN3201522 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 69-317 Log in to see
0.12445667 ABIN3186686 ELISA IF WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12445667 ABIN2497218 WB Rabbit IgG Log in to see Polyclonal
0.12445667 ABIN2497217 IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.12445667 ABIN3186217 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.12445667 ABIN1169326 FACS ELISA WB Rabbit Log in to see Polyclonal
0.12445667 ABIN2405446 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.12445667 ABIN522062 WB Rabbit AA 1-244, full length Log in to see Polyclonal
0.12445667 ABIN948707 WB Mouse AA 1-244, full length Log in to see Polyclonal
0.12445667 ABIN2497219 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal

Top referenced anti-TNFSF11 antibody for Western Blotting

Similar anti-TNFSF11 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 6 Antibodies 32 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 19 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Flow Cytometry (FACS) 28 Antibodies
Functional Studies (Func) 2 Antibodies

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
Epitope Center
(15), (14), (13), (13), (11), (10), (9), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(169), (116), (39), (1), (1), (1), (1)
Host Rabbit
(163), (47), (29), (7), (1)
Clonality
Polyclonal
Conjugate This TNFSF11 antibody is un-conjugated
(26), (12), (6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(173), (107), (46), (44), (28), (22), (21), (12), (10), (10), (9), (5), (5), (3), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-TNFSF11 Antibody

Target Details TNFSF11 Application Details Handling References for anti-TNFSF11 Antibody (ABIN500805) Images
Specificity This antibody detects CD254 / RANKL. It will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Immunoaffinity Chromatography
Immunogen sRANK-L antibody was raised against a 14 amino acid peptide from near the center of Human sRANK-L.
Isotype IgG

Target Details TNFSF11

Product Details anti-TNFSF11 Antibody Application Details Handling References for anti-TNFSF11 Antibody (ABIN500805) Images back to top
Antigen
Alternative Name CD254 / RANKL (TNFSF11 Antibody Abstract)
Background The receptor activator of NF-kappaB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
Gene ID 8600
NCBI Accession NP_003692
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Handling References for anti-TNFSF11 Antibody (ABIN500805) Images back to top
Application Notes ELISA. Western blot: 0.25 - 0.5 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details References for anti-TNFSF11 Antibody (ABIN500805) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-TNFSF11 Antibody (ABIN500805)

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Handling Images back to top
Background publications

Bharti, Takada, Shishodia, Aggarwal: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004

Weitzmann, Cenci, Rifas, Brown, Pacifici: "Interleukin-7 stimulates osteoclast formation by up-regulating the T-cell production of soluble osteoclastogenic cytokines." in: Blood, Vol. 96, Issue 5, pp. 1873-8, 2000

Kong, Yoshida, Sarosi, Tan, Timms, Capparelli, Morony, Oliveira-dos-Santos, Van, Itie, Khoo, Wakeham, Dunstan, Lacey, Mak, Boyle, Penninger: "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis." in: Nature, Vol. 397, Issue 6717, pp. 315-23, 1999

Wong, Rho, Arron, Robinson, Orlinick, Chao, Kalachikov, Cayani, Bartlett, Frankel, Lee, Choi: "TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25190-4, 1997

Images

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Handling References for anti-TNFSF11 Antibody (ABIN500805) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TNFSF11 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 11) (Center) (ABIN500805) Immunohistochemistry of sRANK-L in human liver tissue with this product at 5 μg/ml.
Western Blotting (WB) image for anti-TNFSF11 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 11) (Center) (ABIN500805) Western blot analysis of sRANK-L in rat liver tissue lysate with this product at (A) ...