anti-Rat (Rattus) TNFSF11 antibody for ELISA

Recommended TNFSF11 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
  • TNFSF11
  • BTF3L1
  • HUMBTFB
  • OCIF
  • OPG
  • TNFRSF11B
  • CD254
  • ODF
  • OPGL
  • OPTB2
  • RANKL
  • TRANCE
  • hRANKL2
  • sOdf
  • Ly109l
  • Trance
  • Opg
  • tumor necrosis factor (ligand) superfamily, member 11
  • basic transcription factor 3 pseudogene 11
  • tumor necrosis factor receptor superfamily, member 11b
  • TNFSF11
  • BTF3P11
  • Tnfsf11
  • Tnfrsf11b
  • TNFRSF11B
Epitope
AA 143-316
52
15
15
15
11
10
8
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
252
185
80
3
2
1
1
1
Host
Rabbit
197
97
37
8
1
Clonality
Polyclonal
Conjugate
This TNFSF11 antibody is un-conjugated
28
13
10
9
6
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
254
120
97
81
53
37
26
26
23
14
14
7
5
4
4
3
3
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN964789
$ 379.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3187907 ELISA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN3186686 ELISA IF WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4249074 ELISA Biotin Mouse IgG1 kappa AA 1-317 Log in to see 12A668 9
1 ABIN4886751 ELISA WB Rabbit IgG AA 141-318 Log in to see Polyclonal
1 ABIN1103903 ELISA WB Rabbit IgG AA 143-316 Log in to see Polyclonal
1 ABIN1585704 IP IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN4361912 ELISA IHC IHC (p) WB Rabbit Center Log in to see Polyclonal
1 ABIN2575040 ELISA WB Rabbit IgG AA 106-240 Log in to see Polyclonal
1 ABIN5633448 ELISA WB Rabbit IgG AA 268-317 Log in to see Polyclonal
1 ABIN3201524 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN2923532 ELISA IF/ICC IHC WB Rabbit AA 106-240 Log in to see Polyclonal

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
Epitope AA 143-316
(52), (15), (15), (15), (11), (10), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(252), (185), (80), (3), (2), (1), (1), (1)
Host Rabbit
(197), (97), (37), (8), (1)
Clonality
Polyclonal
Conjugate This TNFSF11 antibody is un-conjugated
(28), (13), (10), (9), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(254), (120), (97), (81), (53), (37), (26), (26), (23), (14), (14), (7), (5), (4), (4), (3), (3), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-TNFSF11 Antibody

Target Details TNFSF11 Application Details Handling Images
Specificity Anti-RANK L antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97-99% homology, there is a chance of cross-reactivity to RANKL from rat, based on 89-94% homology, a chance of cross-reactivity to horse RANKL, on 87-93% homology, to human RANKL-2 and RANKL isoform 1 and to orangutan RANKL, and on 86-93% homology, a chance of cross-reactivity to dog, cattle, sheep, pig, chimpanzee, and macaque RANKL. Cross-reactivity with RANKL from other sources has not been determined.
Characteristics Anti-RANK L antibody is useful in studying the regulating NF-kappaB activation. Secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE, ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.
Purification purified
Sterility Sterile filtered
Immunogen This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details TNFSF11

Product Details anti-TNFSF11 Antibody Application Details Handling Images back to top
Antigen
Background Anti-RANK L antibody is useful in studying the regulating NF-κB activation. Secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE; ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.
Synonyms: Receptor activator of nuclear factor kappa B, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNF11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254
Gene ID 21943
NCBI Accession NP_035743
UniProt O35235
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Handling Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting.. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kDa in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.
Comment

Gene Name: Tnfsf11

Restrictions For Research Use only

Handling

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
Expiry Date 6 months

Images

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 143-316) antibody (ABIN964789) Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL ...