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Human TNFSF13B ELISA Kit

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Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
  • BAFF
  • BLyS
  • BLYS
  • CD257
  • D8Ertd387e
  • DTL
  • RGD1561519
  • TALL-1
  • TALL1
  • THANK
  • TNFSF13B
  • TNFSF20
  • zTNF4
  • ZTNF4
Epitope
AA 134-285
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
18
5
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921083
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN417213 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see 1
0.30628014 ABIN1113646 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.30628014 ABIN1568574 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.30628014 ABIN2708142 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.30628014 ABIN2115103 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012155735 ABIN365023 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Log in to see 3
0.0012155735 ABIN454497 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012155735 ABIN1112559 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN4219262 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 16-1000 pg/mL 16 pg/mL Log in to see
0.0012155735 ABIN2745441 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.6-500 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2042354 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2685748 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN3045591 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN1846857 Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN1033739 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Body Fluids Log in to see
0.0012155735 ABIN2541889 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155735 ABIN2115884 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2945508 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2541886 Tissue Homogenate, Cell Lysate, Biological Fluids Log in to see
0.0012155735 ABIN1719957 Log in to see

Top referenced TNFSF13B ELISA Kit for ELISA

Similar TNFSF13B ELISA Kits

Application / Reactivity Human
ELISA 21 ELISA Kits

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
Epitope AA 134-285
Reactivity Human
Kits with alternative reactivity to:
(21), (18), (5), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 5 references available
Supplier Log in to see

Product Details TNFSF13B ELISA Kit

Target details Application Details Handling References for TNFSF13B Kit (ABIN921083) Images
Purpose For quantitative detection of human BAFF in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human BAFF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human BAFF antibod - 1
  • Lyophilized recombinant human BAFF standar - 10ng/tubex2
  • Biotinylated anti- human BAFF antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A134-L285

Target details

Product Details TNFSF13B ELISA Kit Application Details Handling References for TNFSF13B Kit (ABIN921083) Images back to top
Antigen
Alternative Name TNFSF13B (TNFSF13B ELISA Kit Abstract)
Background BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.
Gene ID 10673
UniProt Q9Y275
Research Area Cell Cycle, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details TNFSF13B ELISA Kit Target details Handling References for TNFSF13B Kit (ABIN921083) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A134-L285) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BAFF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human BAFF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BAFF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 400, Standard deviation: 27.2, CV(%): 6.8
  • Sample 2: n=16, Mean(pg/ml): 950, Standard deviation: 71.25, CV(%): 7.5
  • Sample 3: n=16, Mean(pg/ml): 2108, Standard deviation: 90.6, CV(%): 4.3,
  • Sample 1: n=24, Mean(pg/ml): 425, Standard deviation: 39.1, CV(%): 9.2
  • Sample 2: n=24, Mean(pg/ml): 1093, Standard deviation: 108.2, CV(%): 9.9
  • Sample 3: n=24, Mean(pg/ml): 2357, Standard deviation: 228.6, CV(%): 9.7
Restrictions For Research Use only

Handling

Product Details TNFSF13B ELISA Kit Target details Application Details References for TNFSF13B Kit (ABIN921083) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFSF13B Kit (ABIN921083)

Product Details TNFSF13B ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Gözmen, Karapnar, Tüfekçi, Vergin, Yüksel, Irken, Oren: "B-cell-activating factor, a proliferation inducing ligand and co-stimulatory molecules in the pathogenesis of immune thrombocytopenia in childhood." in: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 2014

Background publications

Lai Kwan Lam, King Hung Ko, Zheng, Lu: "Local BAFF gene silencing suppresses Th17-cell generation and ameliorates autoimmune arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 39, pp. 14993-8, 2008

Krumbholz, Theil, Derfuss, Rosenwald, Schrader, Monoranu, Kalled, Hess, Serafini, Aloisi, Wekerle, Hohlfeld, Meinl: "BAFF is produced by astrocytes and up-regulated in multiple sclerosis lesions and primary central nervous system lymphoma." in: The Journal of experimental medicine, Vol. 201, Issue 2, pp. 195-200, 2005

Schiemann, Gommerman, Vora, Cachero, Shulga-Morskaya, Dobles, Frew, Scott: "An essential role for BAFF in the normal development of B cells through a BCMA-independent pathway." in: Science (New York, N.Y.), Vol. 293, Issue 5537, pp. 2111-4, 2001

Schneider, MacKay, Steiner, Hofmann, Bodmer, Holler, Ambrose, Lawton, Bixler, Acha-Orbea, Valmori, Romero, Werner-Favre, Zubler, Browning, Tschopp: "BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1747-56, 1999

Images

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Supplier Images
ELISA image for TNFSF13B ELISA Kit (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b) (ABIN921083) Human BAFF PicoKine ELISA Kit standard curve