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Mouse (Murine) TNFSF13B ELISA Kit

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Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
  • BAFF
  • BLyS
  • BLYS
  • CD257
  • D8Ertd387e
  • DTL
  • RGD1561519
  • TALL-1
  • TALL1
  • THANK
  • TNFSF13B
  • TNFSF20
  • zTNF4
  • ZTNF4
Epitope
AA 127-309
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
22
17
5
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
46.9-3000 pg/mL
Minimum Detection Limit
46.9 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921084
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30697846 ABIN415813 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.30697846 ABIN1113641 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.30697846 ABIN2708459 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.30697846 ABIN2115102 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012147224 ABIN367653 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
0.0012147224 ABIN1568575 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012147224 ABIN1112558 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012147224 ABIN2042355 Sandwich ELISA 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012147224 ABIN2685749 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012147224 ABIN1846858 Serum, Plasma, Biological Fluids 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012147224 ABIN1164364 78-5000 pg/mL 78 pg/mL Log in to see
0.0012147224 ABIN2541890 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012147224 ABIN2115883 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012147224 ABIN2945509 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012147224 ABIN2112143 Competition ELISA Log in to see
0.0012147224 ABIN1720349 Log in to see

Top referenced TNFSF13B ELISA Kit for ELISA

Similar TNFSF13B ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 17 ELISA Kits

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) ELISA Kits
Epitope AA 127-309
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(22), (17), (5), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 46.9-3000 pg/mL
Minimum Detection Limit 46.9 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TNFSF13B ELISA Kit

Target details Application Details Handling References for TNFSF13B Kit (ABIN921084) Images
Purpose For quantitative detection of mouse BAFF in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse BAFF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse BAFF antibod - 1
  • Lyophilized recombinant mouse BAFF standar - 10ng/tubex2
  • Biotinylated anti- mouse BAFF antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A127-L309

Target details

Product Details TNFSF13B ELISA Kit Application Details Handling References for TNFSF13B Kit (ABIN921084) Images back to top
Antigen
Alternative Name TNFSF13B (TNFSF13B ELISA Kit Abstract)
Background B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.
Gene ID 24099
UniProt Q3KP92
Research Area Cell Cycle, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details TNFSF13B ELISA Kit Target details Handling References for TNFSF13B Kit (ABIN921084) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A127-L309) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BAFF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL mouse BAFF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse BAFF standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 139, Standard deviation: 4.9, CV(%): 3.5
  • Sample 2: n=16, Mean(pg/ml): 312, Standard deviation: 11.5, CV(%): 3.7
  • Sample 3: n=16, Mean(pg/ml): 1508, Standard deviation: 58.8, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 145, Standard deviation: 14.4, CV(%): 9.9
  • Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 33.6, CV(%): 9.5
  • Sample 3: n=24, Mean(pg/ml): 1397, Standard deviation: 124.3, CV(%): 8.9
Restrictions For Research Use only

Handling

Product Details TNFSF13B ELISA Kit Target details Application Details References for TNFSF13B Kit (ABIN921084) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFSF13B Kit (ABIN921084)

Product Details TNFSF13B ELISA Kit Target details Application Details Handling Images back to top
Background publications

Oren, Li, Volovik, Morris, Dharia, Das, Galperina, Gentz, Arnold: "Structural basis of BLyS receptor recognition." in: Nature structural biology, Vol. 9, Issue 4, pp. 288-92, 2002

Schneider, MacKay, Steiner, Hofmann, Bodmer, Holler, Ambrose, Lawton, Bixler, Acha-Orbea, Valmori, Romero, Werner-Favre, Zubler, Browning, Tschopp: "BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1747-56, 1999

Images

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Supplier Images
ELISA image for TNFSF13B ELISA Kit (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b) (ABIN921084) Mouse BAFF PicoKine ELISA Kit standard curve