anti-Human TRADD antibody for Western Blotting

Recommended TRADD Antibody (supplied by: Log in to see )

Antigen
TNFRSF1A-Associated Via Death Domain (TRADD) Antibodies
  • TRADD
  • Hs.89862
  • 9130005N23Rik
  • AA930854
  • wu:fc59e02
  • zgc:103556
  • tradd
  • TNFRSF1A-associated via death domain
  • conjugal transfer protein D
  • tnfrsf1a-associated via death domain
  • tnfrsf1a-associated via death domain protein
  • TRADD
  • LOC100337888
  • traDd
  • Tradd
  • tradd
  • LOC100136668
Reactivity
Human, Mouse (Murine), Rat (Rattus)
119
37
26
6
4
3
3
2
Host
Rabbit
97
32
Clonality
Polyclonal
Conjugate
This TRADD antibody is un-conjugated
5
5
3
3
3
3
3
3
3
2
2
2
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
103
55
44
43
30
27
18
11
5
2
1
1
1
1
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Catalog No. ABIN223257
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2792121 WB Rabbit Middle Region Log in to see Polyclonal 1
3.2182703 ABIN955307 EIA FACS IF IHC (p) WB Rabbit Ig AA 133-159, Middle Region Log in to see Polyclonal 2
3.2182703 ABIN407974 IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.2182703 ABIN968045 IF IP IHC WB Mouse IgG1 AA 163-312 Log in to see 37-TRADD 5
3.2182703 ABIN968046 IF IP IHC WB Mouse IgG1 AA 163-312 Log in to see 37-TRADD 5
3.2182703 ABIN1533447 IHC ELISA WB Rabbit IgG AA 251-300 Log in to see Polyclonal
3.2182703 ABIN967541 WB Mouse IgG1 AA 14-62 Log in to see B36-2 3
3.2182703 ABIN655903 FACS IF IHC (p) WB Rabbit Ig AA 126-151, Center Log in to see Polyclonal
3.2182703 ABIN3187319 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.2182703 ABIN2855906 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.2182703 ABIN5611647 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.2182703 ABIN2705304 IHC WB Rabbit C-Term Log in to see Polyclonal
3.2182703 ABIN1875189 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN396065 WB Mouse IgG2a kappa AA 203-313 Log in to see 3G1
3.2182703 ABIN3021542 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN1860856 ICC IHC IP WB Rabbit IgG AA 61-297 Log in to see Polyclonal
3.2182703 ABIN522159 IF ELISA WB Mouse IgG2a kappa AA 203-312, partial Log in to see 3G1
3.2182703 ABIN2403960 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN522158 IF WB Rabbit AA 1-312, full length Log in to see Polyclonal
3.2182703 ABIN926870 WB Rabbit Middle Region Log in to see Polyclonal

General

Antigen TNFRSF1A-Associated Via Death Domain (TRADD) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(119), (37), (26), (6), (4), (3), (3), (2)
Host Rabbit
(97), (32)
Clonality
Polyclonal
Conjugate This TRADD antibody is un-conjugated
(5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(103), (55), (44), (43), (30), (27), (18), (11), (5), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-TRADD Antibody

Target Details TRADD Application Details Handling References for anti-TRADD antibody (ABIN223257) Images
Blocking Peptide Blocking peptide for this product available: ABIN412952
Specificity The antibody recognizes 34 kDa TRADD in samples from human, mouse, and rat origins.
Reactivity to other species has not been tested.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 300 of human TRADD
Isotype IgG

Target Details TRADD

Product Details anti-TRADD Antibody Application Details Handling References for anti-TRADD antibody (ABIN223257) Images back to top
Antigen
Alternative Name TRADD (TRADD Antibody Abstract)
Background TRADD (TNF-R1-associated death domain) is a 34 kDa protein that associates with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFκB activation and apoptosis in the absence of TNF.
Synonyms: MGC11078 , Hs.89862 , TNFR1
Gene ID 8717
UniProt Q15628
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Hepatitis C

Application Details

Product Details anti-TRADD Antibody Target Details TRADD Handling References for anti-TRADD antibody (ABIN223257) Images back to top
Application Notes Western blotting (0.5-4 μg/mL). Based on researchers feedback, it also works well in immunoprecipitation (10-20 μg/mL) and Immunohistochemistry (15-25 μg/mL). However, the optimal concentrations should be determined individually. The antibody recognizes 34 kDa TRADD in samples from human, mouse, and rat origins. Reactivity to other species has not been tested. Mouse small intestine tissue lysate can be used as a positive control.
Restrictions For Research Use only

Handling

Product Details anti-TRADD Antibody Target Details TRADD Application Details References for anti-TRADD antibody (ABIN223257) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-TRADD antibody (ABIN223257)

Product Details anti-TRADD Antibody Target Details TRADD Application Details Handling Images back to top
Product cited in:

Chen, Texada, Duggan, Liang, Reden, Kooragayala, Langford: "Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells." in: The Journal of biological chemistry, Vol. 280, Issue 23, pp. 22425-36, 2005

Images

Product Details anti-TRADD Antibody Target Details TRADD Application Details Handling References for anti-TRADD antibody (ABIN223257) back to top
Supplier Images
Western Blotting (WB) image for anti-TNFRSF1A-Associated Via Death Domain (TRADD) antibody (ABIN223257) Western blot analysis of TRADD expression in mouse small intestine