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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||10 Antibodies||18 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||27 Antibodies|
|Antigen||TNF Receptor-Associated Factor 3 (TRAF3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TRAF3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-TRAF3 AntibodyTarget Details TRAF3 Application Details Handling References for anti-TRAF3 Antibody (ABIN1003296) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN1003903|
|Purification||Affinity chromatography purified via peptide column.|
|Immunogen||Rabbit polyclol TRAF3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRAF3 (Genbank accession No. NP_663777).|
Target Details TRAF3Product Details anti-TRAF3 Antibody Application Details Handling References for anti-TRAF3 Antibody (ABIN1003296) Images back to top
|Alternative Name||TNF Receptor Associated Factor 3 (TRAF3 Antibody Abstract)|
|Background||Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-kappaB. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.|
|Pathways||NF-kappaB Signaling, Apoptosis, TLR Signaling|
Application DetailsProduct Details anti-TRAF3 Antibody Target Details TRAF3 Handling References for anti-TRAF3 Antibody (ABIN1003296) Images back to top
|Application Notes||TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-TRAF3 Antibody Target Details TRAF3 Application Details References for anti-TRAF3 Antibody (ABIN1003296) Images back to top
|Buffer||Antibody is supplied in PBS containing 0.02% sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.|
|Expiry Date||12 months|
References for anti-TRAF3 Antibody (ABIN1003296)Product Details anti-TRAF3 Antibody Target Details TRAF3 Application Details Handling Images back to top
|Product cited in:||
van Eyndhoven, Gamper, Cho et al.: "TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF-kappaB activation." in: Molecular immunology, Vol. 36, Issue 10, pp. 647-58, 1999 (PubMed).
Arch, Gedrich, Thompson: "Tumor necrosis factor receptor-associated factors (TRAFs)--a family of adapter proteins that regulates life and death." in: Genes & development, Vol. 12, Issue 18, pp. 2821-30, 1998 (PubMed).
Mosialos, Birkenbach, Yalamanchili et al.: "The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family." in: Cell, Vol. 80, Issue 3, pp. 389-99, 1995 (PubMed).
Cheng, Cleary, Ye et al.: "Involvement of CRAF1, a relative of TRAF, in CD40 signaling." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1494-8, 1995 (PubMed).