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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||3 Antibodies||2 Antibodies|
|Western Blotting (WB)||126 Antibodies||70 Antibodies||56 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||22 Antibodies||19 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies||22 Antibodies||17 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies||24 Antibodies||27 Antibodies|
|Antigen||TNF Receptor-Associated Factor 6 (TRAF6) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TRAF6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-TRAF6 AntibodyTarget Details TRAF6 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Mfn2 protein. (region surrounding His154)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details TRAF6Product Details anti-TRAF6 Antibody Application Details Handling Images back to top
|Alternative Name||TRAF6 / RNF85 (TRAF6 Antibody Abstract)|
|Background||Tumor necrosis factor receptor-associated factor 6 (TRAF6) regulates adaptive immunity, innate immunity and bone metabolism. TRAF6 is a ubiquitin (Ub) ligase that mediates the activation of protein kinases, such as transforming growth factor beta-activated kinase (TAK1) and IkappaB kinase (IKK), by catalyzing the formation of a unique polyubiquitin chain linked through Lys 63 of Ub. TRAF6 is essential for activating NFκB signaling pathway in response to interleukin-1 and Toll-like receptor ligands. The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination and activating NFkappaB signaling pathway. TRAF6 interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways.Synonyms: Interleukin-1 signal transducer, RING finger protein 85, TNF receptor-associated factor 6|
|Molecular Weight||approx. 58 kDa|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity|
Application DetailsProduct Details anti-TRAF6 Antibody Target Details TRAF6 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TRAF6 Antibody Target Details TRAF6 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-TRAF6 Antibody Target Details TRAF6 Application Details Handling back to top