You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Baboon Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human, Monkey, Pig (Porcine), Baboon
705
308
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Mouse
538
394
157
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to FITC
147
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
633
587
319
208
120
100
99
86
80
63
56
56
25
25
22
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN263070
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010826217 ABIN263069 ICC FACS IF ELISA Biotin Mouse IgG1 kappa Log in to see MAb11
0.0010826217 ABIN263068 ICC FACS IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see MAb11

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Pig (Porcine) Monkey
ELISA 42 Antibodies 16 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 8 Antibodies
Flow Cytometry (FACS) 15 Antibodies 13 Antibodies
Functional Studies (Func) 21 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 28 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Monkey, Pig (Porcine), Baboon
(705), (308), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(538), (394), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to FITC
(147), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(633), (587), (319), (208), (120), (100), (99), (86), (80), (63), (56), (56), (25), (25), (22), (22), (15), (14), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity TNF- alpha (MAb11)
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Swine (Pig, Porcine).
Purification Affinity purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principallyCD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomerichuman TNF- alpha is a 157 amino acid protein (non-glycosylated) with a reportedmolecular weight of 17 kD. TNF- alpha forms multimeric complexes, stable trimers aremost common in solution. A 26 kD membrane form of TNF- alpha has also beendescribed. TNF- alpha binding to surface receptors elicits a wide array of biologicactivities including: cytolysis and cytostasis of many tumor cell lines in vitro,hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhancedchemotaxis and phagocytosis in neutrophils. The MAb11 antibody can neutralize thebioactivity of natural or recombinant TNF- alpha. Alternate Names: anti-Tumor necrosis factor-alpha antibody, anti-Cachectin antibody, anti-Necrosinantibody, anti-Macrophage cytotoxic factor (MCF) antibody, anti-Differentiation inducingfactor (DIF) antibody, anti-TNFSF2 antibody, anti-TNF-a antibody, anti-TNF-alphaantibody.
Gene Symbol: TNF
Gene ID 7124
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes The purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 1 µL/tests
Buffer PBS, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (FITC) (ABIN263070) PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and MAb11 PE